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JoVE Journal Biochemistry
Uso do elemento Alu contendo Minigenes para analisar RNAs circulares
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Uso do elemento Alu contendo Minigenes para analisar RNAs circulares

Article DOI: 10.3791/59760-v • 13:10 min • March 10th, 2020
March 10th, 2020 •
1, 1, 1, 1
1Institute for Molecular and Cellular Biochemistry, University of Kentucky

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Clonamos e analisamos genes de repórteres gerando RNAs circulares. Esses genes de repórter são maiores do que construções para analisar emendas lineares e conter elementos Alu. Para investigar os RNAs circulares, os construtos são transfeccionados em células e o RNA resultante é analisado usando RT-PCR após a remoção do RNA linear.

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Bioquímica Edição 157 RNAs circulares splicing alternativo gene repórter minigene RNA mRNA
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