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Journal / Immunology and Infection / Imagem latente intravital de linfócitos…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Imagem latente intravital de linfócitos intraepithelial no intestino pequeno murine
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Imagem latente intravital de linfócitos intraepithelial no intestino pequeno murine

Article DOI: 10.3791/59853-v • 08:00 min • June 24th, 2019
June 24th, 2019 •
1, 1
1Center for Immunity and Inflammation, Department of Pathology, Immunology and Laboratory Medicine, Rutgers New Jersey Medical School

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Nós descrevemos um método para visualizar o IELS do ltγδ GFP-etiquetado usando a imagem latente intravital do intestino pequeno murino pela microscopia confocal invertida do disco de giro. Esta técnica permite o rastreamento de células ao vivo dentro da mucosa para até 4 h e pode ser usada para investigar uma variedade de interações imunológicas intestinais.

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Imunologia e infecção imunologia célula T linfócitos intraepiteliais mouse intestino delgado imagem intravital microscopia confocal de disco giratório rastreamento de células
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