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Journal / Immunology and Infection / Análisis de SSHHPS Viral del Grupo IV utilizando…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Análisis de SSHHPS Viral del Grupo IV utilizando métodos In Vitro y In Silico
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Análisis de SSHHPS Viral del Grupo IV utilizando métodos In Vitro y In Silico

Article DOI: 10.3791/60421-v • 10:40 min • December 21st, 2019
December 21st, 2019 •
1, 2, 2
1National Center for Advancing Translational Sciences, National Institutes of Health, 2United States Naval Research Laboratory

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Presentamos un protocolo general para identificar tramos cortos de secuencias de proteínas homolopégicos de patógenos de host (SSHHPS) incrustadas en la poliproteína viral. SSHHPS son reconocidos por proteasas virales y dirigen la destrucción dirigida de proteínas huésped específicas por varios virus del Grupo IV.

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Inmunología e infección número 154 enzima ensayo FRET proteína no estructural alfavirus grupo IV proteasa SSHHPS ensayo de gel in vitro acoplamiento
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