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Dextran Labeling and Uptake in Live and Functional Murine Cochlear Hair Cells

Article DOI: 10.3791/60769-v • 05:55 min • February 8th, 2020
February 8th, 2020 •
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1Molecular Physiology and Biophysics Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health

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Hier presenteren we een methode voor het visualiseren van de opname van 3 kDa Texas Red-gelabelde dextran in auditieve haarcellen met functionele mechanotransductiekanalen. Daarnaast kan dextrans van 3-10 kDa worden gebruikt om endocytose in haar en ondersteunende cellen van het orgaan van Corti te bestuderen.

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Bio-engineering Dextran binnenoor haarcellen mechanotransductiekanaal kleurstofopname endocytose intracellulaire blaasjes confocale microscopie
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