JoVE Monthly Highlights: November 2018

1JoVE Content Production

A Wind Tunnel for Odor Mediated Insect Behavioural Assays

Geir K. Knudsen1, Marco Tasin2, Anders Aak3, Gunda Thöming1

Here, we describe the construction and use of a wind tunnel for odor mediated behavioural assays with insects. The wind tunnel design facilitates the release of odor sources by several methods, with and without visual stimuli. Wind tunnel experiments are important methods to identify behaviorally active volatile chemicals.

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Yavuz F. Yazicioglu1,2, Halil I. Aksoylar1,2, Rinku Pal1,2, Nikolaos Patsoukis1,2, Vassiliki A. Boussiotis1,2

In this study, we present a novel and effective protocol for the isolation of lymphocytes from Peyer's Patches (PPs), which can be subsequently used for in vivo and in vitro functional assays as well as flow cytometric studies of follicular T helper and germinal center B cells.

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

John W. Hickey1,2,3,4, Jonathan P. Schneck2,4

Antigen-specific T cells are difficult to characterize or utilize in therapies due to their extremely low frequency. Herein, we provide a protocol to develop a magnetic particle which can bind to antigen-specific T cells to enrich these cells and then to expand them several hundred-fold for both characterization and therapy.

Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

Neil D. Sanscrainte1, Christy M. Waits2,3, Christopher J. Geden1, Alden S. Estep1,2, James J. Becnel1

This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.