Monitoreo de actina desmontaje con la Microscopía de Time-lapse

Published 11/08/2006
1 Comment

Your institution must subscribe to JoVE's Biology section to access this content.

Fill out the form below to receive a free trial or learn more about access:


Enter your email below to get your free 10 minute trial to JoVE!

By clicking "Submit", you agree to our policies.



Cite this Article

Copy Citation

Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy. J. Vis. Exp. (1), e66, doi:10.3791/66 (2006).

Please note that all translations are automatically generated.

Click here for the english version. For other languages click here.



  1. Construir una cámara de flujo como se muestra en el video. Asegúrese de que el sello de parafina es escaso y el uso de cubreobjetos se lavan.
  2. Incubar actina agente fijador en la cámara para '10.5.
  3. Bloquear no específicas sitios de unión sobre el cubreobjetos con una proteína de bloqueo.
  4. Actina polimeriza dentro de la cámara de flujo en G-actina en la polimerización de amortiguamiento.
  5. Lavado de actina polimerizada por el flujo en exceso de tampón.

Subscription Required. Please recommend JoVE to your librarian.


1 Comment

  1. What is the second pair of shorter parafilm strips for? Why do you polymerize in the chamber and how? G-actin in polymerization buffer. Is't that F-actin? What is the actin binding agent and blocking protein and their buffers and their concentrations?

    Posted by: Anonymous
    July 28, 2009 - 2:41 PM

Post a Question / Comment / Request

You must be signed in to post a comment. Please or create an account.

Video Stats