位相コントラストおよび微分干渉コントラスト(DIC)顕微鏡

Published 8/06/2008
9 Comments
  CITE THIS  SHARE 
Biology
 

Summary

このプロトコルは、位相と微分干渉コントラスト(DIC)顕微鏡の原理と実用化を強調

Cite this Article

Copy Citation

Centonze Frohlich, V. Phase Contrast and Differential Interference Contrast (DIC) Microscopy. J. Vis. Exp. (18), e844, doi:10.3791/844 (2008).

Please note that all translations are automatically generated.

Click here for the english version. For other languages click here.

Abstract

位相差顕微鏡は、多くの場合、透明な、非光吸収、生体試料のコントラストを生成するために使用されます。技術は彼の功績に対してノーベル賞を受けた1942年のゼルニケ、、によって発見された。それは、試料の構造細部のエッジを強調組織細胞、卵、及び胚を含む厚い試料の高分解能の光学切片を提供し、位相のハロー典型的に苦しむしないため、1960年代後半に導入されたDIC顕微鏡は、生物医学研究で親しまれてきた位相コントラスト像の。このプロトコルは、これらの顕微鏡技術の原理と実用化を強調。

Protocol

この実験的なアプローチのための完全なテキストのプロトコルでは、利用可能です細胞生物学におけるカレントプロトコール。

Acknowledgements

位相コントラストとDIC顕微鏡を示すいくつかのビデオクリップは、と題する、別のビデオ-の記事から採取した共生のレイヤーを 。 Joveのは、優雅にレッドベターラボによるこの貢献を認めている。

Comments

9 Comments

  1. how much will each cost

    Reply
    Posted by: Anonymous
    September 15, 2008 - 1:23 PM
  2. The systems vary widely to correlate with your needs.  I would be happy to help you further. 631-547-8501, Ned Jastromb, Product Manager, Nikon Instruments, Inc.

    Reply
    Posted by: Anonymous
    September 16, 2008 - 11:15 AM
  3. Excellent - I'm definitely going to use this for my students, who are from a mixed background (biologists, physicists, chemists, engineers).

    Reply
    Posted by: Anonymous
    September 18, 2008 - 8:40 AM
  4. I've seen that there we're colored images obtained using the DIC microscopy that looks like a 3-D image. Is there an approximation using DIC to measure the depth or height difference by just interpreting color differences?

    Reply
    Posted by: Anonymous
    May 27, 2009 - 7:42 AM
  5. I've seen that there we're colored images obtained using the DIC microscopy that looks like a 3-D image. Is there an approximation using DIC to measure the depth or height difference by just interpreting color differences?

    Reply
    Posted by: Anonymous
    May 27, 2009 - 7:59 AM
  6. In article abstract, the phase-contrast microscopy is claimed to have been invented in 194². This is incorrect. The origins of phase-contrast date back to 1934 (not a microscopy yet) and 1935 (microscopy):

    Zernike F. (1934)
    Diffraction theory of the knife-edge test and its improved form, the phase-contrast method.
    Monthly Notices of the Royal Astronomical Society 94 (5), 377-3­84 (originally published in Dutch).
    (http://esoads.eso.org/journals_service.html)

    Zernike F. (1935)
    Phase-contrast method in microscopical observation (in German).
    Physikalische Zeitschrift 36 (²²/²3): 848-851
    Zeitschrift für Technische Physik 16 (11): 454-457
    (two identical papers)

    Furthermore, the following reports (published when Zernike was only 16 or 17 years old) utilize, in a way, the principle of phase-contrast microscopy, even though only for absorbing (i.e., not phase) objects:

    Rheinberg J. (1904 and 1905)
    On the influence on images of gratings of phase differences amongst their spectra.
    J. Roy. Microsc. Soc.; volumes-not-numbered (Part 4), 388-390; and (Part ²), 15²-155.
    (www.biodiversitylibrary.org/bibliography/7413)

    Conrady A. (1905)
    An experimental proof of phase reversal in diffraction spectra.
    J. Roy. Microsc. Soc.; volume-not-numbered (Part ²), 150-15².
    (www.biodiversitylibrary.org/bibliography/7413)

    Reply
    Posted by: Anonymous
    May 4, 2010 - 11:14 AM
  7. Your presentation is excellent and locked in my neurons , the corresponding text in books do not give that effect

    Reply
    Posted by: Anonymous
    November 12, 2011 - 10:19 PM
  8. Nice video.
    I may be mistaken, but one of the images (around 3:47) shown as DIC appears to be phase contrast.
    Also, when mentioning setting up KŒhler illumination during DIC (around 4:30), the video shows the alignment of phase rings. This is not needed (since you do not need to see the back focal plane of the microscope to establish KŒhler) nor, in fact, what would be observed when setting up KŒhler for DIC (there would be no phase annulus in the condenser if it is set up for DIC).

    Reply
    Posted by: Anonymous
    December 7, 2011 - 10:36 AM
  9. The video explained well what i was trying to learn in detail....
    it is really nice
    Thanks

    Reply
    Posted by: Anonymous
    April 5, 2012 - 2:35 AM

Post a Question / Comment / Request

You must be signed in to post a comment. Please or create an account.

Video Stats