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Articles by Adrianne N. Edwards in JoVE
Culturing and Maintaining Clostridium difficile in an Anaerobic Environment Adrianne N. Edwards1, Jose M. Suárez1, Shonna M. McBride1 1Department of Microbiology and Immunology, Emory University School of Medicine Clostridium difficile is a pathogenic bacterium that is a strict anaerobe and causes antibiotic associated diarrhea (AAD). Here, methods for isolating, culturing and maintaining C. difficile vegetative cells and spores are described. These techniques necessitate an anaerobic chamber, which requires regular maintenance to ensure proper conditions for optimal C. difficile cultivation.