In JoVE (2)
Articles by Alice Brandli in JoVE
Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents Alice Brandli1 1Discipline of Physiology and Bosch Institute, Sydney Medical School, University of Sydney Remote ischemic preconditioning (RIP) is a method of conditioning tissues against damaging stress. We have established a method of remote ischemia at the hind limb, by inflating a sphygmomanometer cuff for 5-10 min. The neuroprotective capabilities of RIP have been demonstrated in a model of retinal degeneration in rodents.
Using the Electroretinogram to Assess Function in the Rodent Retina and the Protective Effects of Remote Limb Ischemic Preconditioning Alice Brandli1, Jonathan Stone1 1Discipline of Physiology and Bosch Institute, Sydney Medical School, University of Sydney The electroretinogram (ERG) is an electrical potential generated by the retina in response to light. This paper describes how to use the ERG to assess retinal function, in dark-adapted rats, and how it can be can be used to assess a neuroprotective intervention, in the present case remote ischemic preconditioning.
Other articles by Alice Brandli on PubMed
Shedding New Light on the Molecular Architecture of Oocytes Using a Combination of Synchrotron Fourier Transform-infrared and Raman Spectroscopic Mapping Analytical Chemistry. Dec, 2008 | Pubmed ID: 18983174 Synchrotron Fourier transform-infrared (FT-IR) and Raman microspectroscopy were applied to investigate changes in the molecular architecture of mouse oocytes and demonstrate the overall morphology of the maturing oocyte. Here we show that differences were identified between immature mouse oocytes at the germinal vesicle (GV) and mature metaphase II (MII) stage when using this technology, without the introduction of any extrinsic markers, labels, or dyes. GV mouse oocytes were found to have a small, centrally located lipid deposit and another larger polar deposit of similar composition. MII oocytes have very large, centrally located lipid deposits. Each lipid deposit for both cell types contains an inner and outer lipid environment that differs in composition. To assess interoocyte variability, line scans were recorded across the diameter of the oocytes and compared from three independent trials (GV, n = 91; MII, n = 172), and the data were analyzed with principal component analysis (PCA). The average spectra and PCA loading plots show distinct and reproducible changes in the CH stretching region that can be used as molecular maturation markers. The method paves the way for developing an independent assay to assess oocyte status during maturation providing new insights into lipid distribution at the single cell level.
Remote Ischemia Influences the Responsiveness of the Retina: Observations in the Rat Investigative Ophthalmology & Visual Science. Apr, 2014 | Pubmed ID: 24569583 Remote ischemic preconditioning (RIP) has been found to be protective of heart and brain against ischemic injury. We have tested the effects of RIP on retinal function using the electroretinogram.