In JoVE (3)
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Articles by Anand Ghanekar in JoVE
Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice Sharif U. Ahmed1, Murtuza Zair1, Kui Chen1, Matthew Iu1, Feng He1, Oyedele Adeyi2, Sean P. Cleary3, Anand Ghanekar1,3 1Toronto General Research Institute, University Health Network, 2Department of Pathology, University Health Network, 3Division of General Surgery, University Health Network Human tumor xenografts in immunodeficient mice are valuable tools to study cancer biology. Specific protocols to generate subcutaneous and intrahepatic xenografts from human hepatocellular carcinoma cells or tumor fragments are described. Liver regeneration induced by partial hepatectomy in recipient mice is presented as a strategy to facilitate intrahepatic engraftment.
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation J. Moritz Kaths1,2, Vinzent N. Spetzler1, Nicolas Goldaracena1, Juan Echeverri1, Kristine S. Louis1, Daniel B. Foltys3, Mari Strempel4, Paul Yip5, Rohan John5, Istvan Mucsi1, Anand Ghanekar1, Darius Bagli6,7, Lisa Robinson2, Markus Selzner1 1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, 2Division of Nephrology, The Hospital for Sick Children, Toronto, 3Department of General, Visceral & Transplant Surgery, University Medical Center Mainz, 4Department of Abdominal, Vascular & Transplant Surgery, Merheim Medical Center Cologne, 5Laboratory Medicine & Pathobiology, Toronto General Hospital, 6Departments of Surgery (Urology) & Physiology, The Hospital for Sick Children, Toronto, 7Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
Heterotopic Renal Autotransplantation in a Porcine Model: A Step-by-Step Protocol J. Moritz Kaths1,2, Juan Echeverri1,3, Nicolas Goldaracena1, Kristine S. Louis1, Paul Yip4, Rohan John4, Istvan Mucsi5, Anand Ghanekar1, Darius Bagli6, Markus Selzner1, Lisa A. Robinson2 1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, 2Division of Nephrology, The Hospital for Sick Children, 3Programa de Doctorat en Medicina, La Universitat Autónoma de Barcelona, 4Laboratory Medicine and Pathobiology, Toronto General Hospital, 5Department of Medicine, Toronto General Hospital, 6Departments of Surgery (Urology) & Physiology, Developmental & Stem Cell Biology, The Hospital for Sick Children Porcine models of organ transplantation provide an important platform to study mechanisms of organ preservation. This article describes a heterotopic porcine renal autotransplantation model, which allows investigating new approaches to improve the outcome of transplantation using marginal kidney grafts.