In JoVE (1)
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Articles by Andrew Richards in JoVE
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices Gordon D. Hoople*1, Andrew Richards*2, Yan Wu2, Albert P. Pisano3, Kun Zhang2 1Department of Engineering, University of San Diego, 2Department of Bioengineering, University of California, San Diego, 3Department of Mechanical and Aerospace Engineering, University of California, San Diego Gel-seq enables researchers to simultaneously prepare libraries for both DNA- and RNA-seq at negligible added cost starting from 100 - 1000 cells using a simple hydrogel device. This paper presents a detailed approach for the fabrication of the device as well as the biological protocol to generate paired libraries.