In JoVE (5)
- The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF
- Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set
- Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy
- Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer
- Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
Other Publications (0)
Articles by Yan Wu in JoVE
The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF Wen Xiong1, Yan Gao1, Xun Cheng1, Charles Martin1, Dongmei Wu1, Shuyuan Yao1, Min-Ju Kim2, Yang Liu1 1Research and Development, Stemgent, 2Product Marketing, Stemgent SC1 functions through dual inhibition of Ras- GAP and ERK1. We tested the function of SC1 in supporting mouse ES cell self-renewal in the absence of LIF and showed that SC1 is able to maintain self-renewal of mouse ES cell cultures.
Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set Dongmei Wu1, Brad Hamilton1, Charles Martin1, Yan Gao1, Mike Ye1, Shuyuan Yao1 1Research and Development, Stemgent We demonstrate the protocol for the generation of induced pluripotent stem cells from human somatic cells using lentivirus-mediated delivery of the human factors Oct4, Sox2, Nanog, and Lin28. Pluripotency was confirmed by morphology and the presence of embryonic stem (ES) cell-specific markers.
Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy Daxiang Li1, Jian Wu1, Yan Bai1, Xiaochen Zhao2, Lijun Liu1 1Department of Biochemistry and Cancer Biology, University of Toledo College of Medicine and Life Sciences, 2Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences We describe a reliable method for isolation of adult mouse cardiomyocytes. This protocol yields a consistent result for the culture of functional adult cardiomyocytes from a variety of genetically modified mice.
Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer Zhihui Xu*1, Yiyan Song*1, Huijun Jiang2, Yan Kong3, Xiaoming Li3, Jin Chen1, Yuan Wu4 1School of Public Health, Nanjing Medical University, 2School of Pharmacy, Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University This protocol describes a general strategy to regenerate commercial arrayed gold microelectrodes equipped for a label-free cell analyzer aimed at saving on the high running costs ofmicrochip-based assays. The regeneration process includes trypsin digestion, rinsing with ethanol and water, and a spinning step, which enables repeated usage of microchips.
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices Gordon D. Hoople*1, Andrew Richards*2, Yan Wu2, Albert P. Pisano3, Kun Zhang2 1Department of Engineering, University of San Diego, 2Department of Bioengineering, University of California, San Diego, 3Department of Mechanical and Aerospace Engineering, University of California, San Diego Gel-seq enables researchers to simultaneously prepare libraries for both DNA- and RNA-seq at negligible added cost starting from 100 - 1000 cells using a simple hydrogel device. This paper presents a detailed approach for the fabrication of the device as well as the biological protocol to generate paired libraries.