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Articles by Anthony J. Sprangers in JoVE
A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo Anthony J. Sprangers1, Brian T. Freeman1, Nicholas A. Kouris1, Brenda M. Ogle2 1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Department of Biomedical Engineering, Materials Science Program, Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison A method to track cell fusion in living organisms over time is described. The approach utilizes Cre-LoxP recombination to induce luciferase expression upon cell fusion. The luminescent signal generated can be detected in living organisms using biophotonic imaging systems with a sensitivity of detection of ˜1,000 cells in peripheral tissues.