In JoVE (1)
Other Publications (1)
Articles by Arong Jung in JoVE
Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors Sungmoo Lee1,2, Hong Hua Piao2, Masoud Sepheri-Rad2, Arong Jung2,3, Uhna Sung2, Yoon-Kyu Song1,4, Bradley J. Baker2 1Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, 2Center for Functional Connectomics, Korea Institute of Science and Technology, 3College of Life Sciences and Biotechnology, Korea University, 4Advanced Institutes of Convergence Technology A method for imaging changes in membrane potential using genetically encoded voltage indicators is described.
Other articles by Arong Jung on PubMed
Linker Length and Fusion Site Composition Improve the Optical Signal of Genetically Encoded Fluorescent Voltage Sensors Neurophotonics. Apr, 2015 | Pubmed ID: 26158002 Several genetically encoded fluorescent sensors of voltage were created by systematically truncating the length of the linker sequence between the voltage-sensing domain and the position of the fluorescent protein, Super Ecliptic A227D. In addition to varying the length, the amino acid composition at the fusion site for the fluorescent protein was modified. Both linker length and amino acid composition affected the size and voltage sensitivity of the optical signal. The truncation mutants revealed a potential structural periodicity with a maximum signal three amino acids from the voltage-sensing domain and another maximum 11 amino acids from the voltage-sensing domain. These results confirm that the linker length and composition can fine tune the size and voltage range of the sensor. The potential periodicity suggests that the orientation of the fluorescent protein could be important for improving the signal size implicating dimerization of the fluorescent protein.