In JoVE (1)
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Articles by Bill Sugden in JoVE
Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy Kathryn Norby1, Ya-Fang Chiu1, Bill Sugden1 1Department of Oncology, University of Wisconsin - Madison A method of observing individual DNA molecules in live cells is described. The technique is based on the binding of a fluorescently tagged lac repressor protein to binding sites engineered into the DNA of interest. This method can be adapted to follow many recombinant DNAs in live cells over time.