In JoVE (1)
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Articles by Brittany Alphonse in JoVE
Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds Hannah A. Strobel1, Elizabeth L. Calamari1, Brittany Alphonse1, Tracy A. Hookway1,2, Marsha W. Rolle1 1Biomedical Engineering, Worcester Polytechnic Institute, 2Gladstone Institute for Cardiovascular Disease This protocol describes a platform for fabricating self-assembled tissue rings in variable sizes using a customized 3D-printed plastic mold. PDMS negatives are cured in the 3D-printed mold; then agarose is cast in the cured PDMS negatives. Cells are seeded into the resulting agarose wells where they aggregate into tissue rings.