Cecilia M. Giachelli Department of Bioengineering, Department of Medicine University of Washington Biography Publications Institution JoVE Articles Cecilia M. Giachelli has not added a biography. If you are Cecilia M. Giachelli and would like to personalize this page please email our Author Liaison for assistance. Publications Comparison of Osteoclast Differentiation Protocols from Human Induced Pluripotent Stem Cells of Different Tissue Origins Stem Cell Research & Therapy. Nov, 2023 | Pubmed ID: 37936199 Comparison of Osteoclast Differentiation Protocols from Human Induced Pluripotent Stem Cells of Different Tissue Origins Research Square. Jul, 2023 | Pubmed ID: 37461708 Dissecting Specific Wnt Components Governing Osteogenic Differentiation Potential by Human Periodontal Ligament Stem Cells Through Interleukin-6 Scientific Reports. Jun, 2023 | Pubmed ID: 37270571 Role of Runx2 in Calcific Aortic Valve Disease in Mouse Models Frontiers in Cardiovascular Medicine. 2021 | Pubmed ID: 34778386 Engineered Osteoclasts Resorb Necrotic Alveolar Bone in Anti-RANKL Antibody-treated Mice Bone. Dec, 2021 | Pubmed ID: 34375732 Adapter Protein RapGEF1 Is Required for ERK1/2 Signaling in Response to Elevated Phosphate in Vascular Smooth Muscle Cells Journal of Vascular Research. 2021 | Pubmed ID: 33951626 Slc20a1/Pit1 and Slc20a2/Pit2 Are Essential for Normal Skeletal Myofiber Function and Survival Scientific Reports. Feb, 2020 | Pubmed ID: 32080237 PiT-2, a Type III Sodium-dependent Phosphate Transporter, Protects Against Vascular Calcification in Mice with Chronic Kidney Disease Fed a High-phosphate Diet Kidney International. Oct, 2018 | Pubmed ID: 30041812 Increased Calcific Aortic Valve Disease in Response to a Diabetogenic, Procalcific Diet in the LDLrApoB Mouse Model Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology. 2018 | Pubmed ID: 29539583 Role of Smooth Muscle Cells in Vascular Calcification: Implications in Atherosclerosis and Arterial Stiffness Cardiovascular Research. Mar, 2018 | Pubmed ID: 29514202 Loss of PiT-2 Results in Abnormal Bone Development and Decreased Bone Mineral Density and Length in Mice Biochemical and Biophysical Research Communications. Jan, 2018 | Pubmed ID: 29133259 Noncanonical Wnts at the Cusp of Fibrocalcific Signaling Processes in Human Calcific Aortic Valve Disease Arteriosclerosis, Thrombosis, and Vascular Biology. Mar, 2017 | Pubmed ID: 28228443 Osteoclast Precursors Do Not Express CD68: Results from CD68 Promoter-driven RANK Transgenic Mice FEBS Letters. Mar, 2017 | Pubmed ID: 28173622 Vascular Calcification in CKD-MBD: Roles for Phosphate, FGF23, and Klotho Bone. Jul, 2017 | Pubmed ID: 27847254 SLC20A2 Deficiency in Mice Leads to Elevated Phosphate Levels in Cerbrospinal Fluid and Glymphatic Pathway-Associated Arteriolar Calcification, and Recapitulates Human Idiopathic Basal Ganglia Calcification Brain Pathology (Zurich, Switzerland). 01, 2017 | Pubmed ID: 26822507 Runx2 Deletion in Smooth Muscle Cells Inhibits Vascular Osteochondrogenesis and Calcification but Not Atherosclerotic Lesion Formation Cardiovascular Research. Nov, 2016 | Pubmed ID: 27671804 Osteopontin Protects Against High Phosphate-induced Nephrocalcinosis and Vascular Calcification Kidney International. May, 2016 | Pubmed ID: 27083280 Slc20a2 Deficiency Results in Fetal Growth Restriction and Placental Calcification Associated with Thickened Basement Membranes and Novel CD13 and Lamininα1 Expressing Cells Reproductive Biology. Mar, 2016 | Pubmed ID: 26952749 Runx2 Expression in Smooth Muscle Cells Is Required for Arterial Medial Calcification in Mice The American Journal of Pathology. Jul, 2015 | Pubmed ID: 25987250 Phosphate Uptake-independent Signaling Functions of the Type III Sodium-dependent Phosphate Transporter, PiT-1, in Vascular Smooth Muscle Cells Experimental Cell Research. Apr, 2015 | Pubmed ID: 25684711 Calcific Aortic Valve Disease: a Consensus Summary from the Alliance of Investigators on Calcific Aortic Valve Disease Arteriosclerosis, Thrombosis, and Vascular Biology. Nov, 2014 | Pubmed ID: 25189570 A Current Understanding of Vascular Calcification in CKD American Journal of Physiology. Renal Physiology. Oct, 2014 | Pubmed ID: 25143458 Loss of PiT-1 Results in Abnormal Endocytosis in the Yolk Sac Visceral Endoderm Mechanisms of Development. Aug, 2014 | Pubmed ID: 25138534 Engineered Biomaterials Control Differentiation and Proliferation of Human-embryonic-stem-cell-derived Cardiomyocytes Via Timed Notch Activation Stem Cell Reports. Mar, 2014 | Pubmed ID: 24672751 An Inducible, Ligand-independent Receptor Activator of NF-κB Gene to Control Osteoclast Differentiation from Monocytic Precursors PloS One. 2013 | Pubmed ID: 24386387 Sodium-dependent Phosphate Cotransporters and Phosphate-induced Calcification of Vascular Smooth Muscle Cells: Redundant Roles for PiT-1 and PiT-2 Arteriosclerosis, Thrombosis, and Vascular Biology. Nov, 2013 | Pubmed ID: 23968976 Vascular Calcification: an Update on Mechanisms and Challenges in Treatment Calcified Tissue International. Oct, 2013 | Pubmed ID: 23456027 Fibroblast Growth Factor 23 is Not Associated with and Does Not Induce Arterial Calcification Kidney International. Jun, 2013 | Pubmed ID: 23389416 Prevascularized Microtemplated Fibrin Scaffolds for Cardiac Tissue Engineering Applications Tissue Engineering. Part A. Apr, 2013 | Pubmed ID: 23317311 Osteopontin Mediates Macrophage Chemotaxis Via α4 and α9 Integrins and Survival Via the α4 Integrin Journal of Cellular Biochemistry. May, 2013 | Pubmed ID: 23192608 Smooth Muscle Cell Plasticity: Fact or Fiction? Circulation Research. Jan, 2013 | Pubmed ID: 23093573 High Phosphate Feeding Promotes Mineral and Bone Abnormalities in Mice with Chronic Kidney Disease Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association. Jan, 2013 | Pubmed ID: 23045434 RANKL Enhances Macrophage Paracrine Pro-calcific Activity in High Phosphate-treated Smooth Muscle Cells: Dependence on IL-6 and TNF-α Journal of Vascular Research. 2012 | Pubmed ID: 22948607 Vitamin D Receptor Agonists Increase Klotho and Osteopontin While Decreasing Aortic Calcification in Mice with Chronic Kidney Disease Fed a High Phosphate Diet Kidney International. Dec, 2012 | Pubmed ID: 22932118 Engineering Biomaterials to Integrate and Heal: the Biocompatibility Paradigm Shifts Biotechnology and Bioengineering. Aug, 2012 | Pubmed ID: 22592568 Sources of Cells That Contribute to Atherosclerotic Intimal Calcification: an in Vivo Genetic Fate Mapping Study Cardiovascular Research. Jun, 2012 | Pubmed ID: 22436847 Combination of Sleeping Beauty Transposition and Chemically Induced Dimerization Selection for Robust Production of Engineered Cells Nucleic Acids Research. Jun, 2012 | Pubmed ID: 22402491 Arterial Calcification in Chronic Kidney Disease: Key Roles for Calcium and Phosphate Circulation Research. Sep, 2011 | Pubmed ID: 21885837 Direct Effects of Phosphate on Vascular Cell Function Advances in Chronic Kidney Disease. Mar, 2011 | Pubmed ID: 21406295 Elastin Degradation and Vascular Smooth Muscle Cell Phenotype Change Precede Cell Loss and Arterial Medial Calcification in a Uremic Mouse Model of Chronic Kidney Disease The American Journal of Pathology. Feb, 2011 | Pubmed ID: 21281809 Matrix Remodeling in Vascular Calcification Associated with Chronic Kidney Disease Journal of the American Society of Nephrology : JASN. Oct, 2010 | Pubmed ID: 20798261 Phosphate and Vascular Calcification: Emerging Role of the Sodium-dependent Phosphate Co-transporter PiT-1 Thrombosis and Haemostasis. Sep, 2010 | Pubmed ID: 20664908 Runx2/Cbfa1, but Not Loss of Myocardin, is Required for Smooth Muscle Cell Lineage Reprogramming Toward Osteochondrogenesis Journal of Cellular Biochemistry. Jul, 2010 | Pubmed ID: 20564193 Characterization of Mandibular Bone in a Mouse Model of Chronic Kidney Disease Journal of Periodontology. Feb, 2010 | Pubmed ID: 20151810 Vitamin D Receptor Activators Induce an Anticalcific Paracrine Program in Macrophages: Requirement of Osteopontin Arteriosclerosis, Thrombosis, and Vascular Biology. Feb, 2010 | Pubmed ID: 19948844 Discoidin Domain Receptor-1 Deficiency Attenuates Atherosclerotic Calcification and Smooth Muscle Cell-mediated Mineralization The American Journal of Pathology. Dec, 2009 | Pubmed ID: 19893047 Generation of Mouse Conditional and Null Alleles of the Type III Sodium-dependent Phosphate Cotransporter PiT-1 Genesis (New York, N.Y. : 2000). Dec, 2009 | Pubmed ID: 19882669 The Role of Osteopontin in Inflammatory Processes Journal of Cell Communication and Signaling. Dec, 2009 | Pubmed ID: 19798593 Immobilization of Alkaline Phosphatase on Microporous Nanofibrous Fibrin Scaffolds for Bone Tissue Engineering Biomaterials. Sep, 2009 | Pubmed ID: 19501906 Phosphate Feeding Induces Arterial Medial Calcification in Uremic Mice: Role of Serum Phosphorus, Fibroblast Growth Factor-23, and Osteopontin Kidney International. Jun, 2009 | Pubmed ID: 19322138 Smooth Muscle Cells Give Rise to Osteochondrogenic Precursors and Chondrocytes in Calcifying Arteries Circulation Research. Mar, 2009 | Pubmed ID: 19197075 The Emerging Role of Phosphate in Vascular Calcification Kidney International. May, 2009 | Pubmed ID: 19145240 Osteoprotegerin and RANKL Differentially Regulate Angiogenesis and Endothelial Cell Function Angiogenesis. 2009 | Pubmed ID: 19105036 Methods to Promote Notch Signaling at the Biomaterial Interface and Evaluation in a Rafted Organ Culture Model Journal of Biomedical Materials Research. Part A. Nov, 2009 | Pubmed ID: 18985776 Role of Carbonic Anhydrase II in Ectopic Calcification Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology. 2009 | Pubmed ID: 18402839 Microporous Nanofibrous Fibrin-based Scaffolds for Bone Tissue Engineering Biomaterials. Oct, 2008 | Pubmed ID: 18640716 Crosslinking of an Oesophagus Acellular Matrix Tissue Scaffold Journal of Tissue Engineering and Regenerative Medicine. Aug, 2008 | Pubmed ID: 18618611 The Influence of Surface Mineral and Osteopontin on the Formation and Function of Murine Bone Marrow-derived Osteoclasts Journal of Materials Science. Materials in Medicine. Oct, 2008 | Pubmed ID: 18483788 BMP-2 Promotes Phosphate Uptake, Phenotypic Modulation, and Calcification of Human Vascular Smooth Muscle Cells Atherosclerosis. Aug, 2008 | Pubmed ID: 18179800 A Fibrinogen-based Precision Microporous Scaffold for Tissue Engineering Biomaterials. Dec, 2007 | Pubmed ID: 17765302 Osteopontin: a Multifunctional Molecule Regulating Chronic Inflammation and Vascular Disease Arteriosclerosis, Thrombosis, and Vascular Biology. Nov, 2007 | Pubmed ID: 17717292 Delivery of Non-viral Gene Carriers from Sphere-templated Fibrin Scaffolds for Sustained Transgene Expression Biomaterials. Nov, 2007 | Pubmed ID: 17675152 Sodium-dependent Phosphate Cotransporters and Vascular Calcification Current Opinion in Nephrology and Hypertension. Jul, 2007 | Pubmed ID: 17565274 Mechanisms of Vascular Calcification Advances in Chronic Kidney Disease. Jan, 2007 | Pubmed ID: 17200044 Molecular Mediators of Alphavbeta3-induced Endothelial Cell Survival Journal of Vascular Research. 2006 | Pubmed ID: 16888388 Osteoprotegerin Inactivation Accelerates Advanced Atherosclerotic Lesion Progression and Calcification in Older ApoE-/- Mice Arteriosclerosis, Thrombosis, and Vascular Biology. Sep, 2006 | Pubmed ID: 16840715 Mimicking Cell-cell Interactions at the Biomaterial-cell Interface for Control of Stem Cell Differentiation Journal of Biomedical Materials Research. Part A. Oct, 2006 | Pubmed ID: 16758464 Development of an Esophagus Acellular Matrix Tissue Scaffold Tissue Engineering. Feb, 2006 | Pubmed ID: 16548690 Role of the Sodium-dependent Phosphate Cotransporter, Pit-1, in Vascular Smooth Muscle Cell Calcification Circulation Research. Apr, 2006 | Pubmed ID: 16527991 Arteriosclerosis, Calcium Phosphate Deposition and Cardiovascular Disease in Uremia: Current Concepts at the Bench Current Opinion in Nephrology and Hypertension. Nov, 2005 | Pubmed ID: 16205469 Activation of IKKbeta by Glucose is Necessary and Sufficient to Impair Insulin Signaling and Nitric Oxide Production in Endothelial Cells Journal of Molecular and Cellular Cardiology. Aug, 2005 | Pubmed ID: 15978611 The Role of Osteopontin in Foreign Body Giant Cell Formation Biomaterials. Oct, 2005 | Pubmed ID: 15949549 Controlling Osteopontin Orientation on Surfaces to Modulate Endothelial Cell Adhesion Journal of Biomedical Materials Research. Part A. Jul, 2005 | Pubmed ID: 15920735 Esophageal Epithelial Cell Interaction with Synthetic and Natural Scaffolds for Tissue Engineering Biomaterials. Nov, 2005 | Pubmed ID: 15913763 Calcification of Advanced Atherosclerotic Lesions in the Innominate Arteries of ApoE-deficient Mice: Potential Role of Chondrocyte-like Cells Arteriosclerosis, Thrombosis, and Vascular Biology. Jul, 2005 | Pubmed ID: 15845913 Regulation of Vascular Calcification: Roles of Phosphate and Osteopontin Circulation Research. Apr, 2005 | Pubmed ID: 15831823 Smooth Muscle Cells Deficient in Osteopontin Have Enhanced Susceptibility to Calcification in Vitro Cardiovascular Research. May, 2005 | Pubmed ID: 15820201 Mitigation of Ectopic Calcification in Osteopontin-deficient Mice by Exogenous Osteopontin Calcified Tissue International. Apr, 2005 | Pubmed ID: 15812576 Free Fatty Acid Impairment of Nitric Oxide Production in Endothelial Cells is Mediated by IKKbeta Arteriosclerosis, Thrombosis, and Vascular Biology. May, 2005 | Pubmed ID: 15731493 Src Kinase Activity is Required for Integrin AlphaVbeta3-mediated Activation of Nuclear Factor-kappaB The Journal of Biological Chemistry. Apr, 2005 | Pubmed ID: 15695822 Vascular Calcification Mechanisms Journal of the American Society of Nephrology : JASN. Dec, 2004 | Pubmed ID: 15579497 The CC Chemokine Ligand, CCL2/MCP1, Participates in Macrophage Fusion and Foreign Body Giant Cell Formation The American Journal of Pathology. Dec, 2004 | Pubmed ID: 15579457 Elevated Extracellular Calcium Levels Induce Smooth Muscle Cell Matrix Mineralization in Vitro Kidney International. Dec, 2004 | Pubmed ID: 15569318 Mechanisms of Vascular Calcification in Uremia Seminars in Nephrology. Sep, 2004 | Pubmed ID: 15490398 Hyaluronic Acid Grafting Mitigates Calcification of Glutaraldehyde-fixed Bovine Pericardium Journal of Biomedical Materials Research. Part A. Aug, 2004 | Pubmed ID: 15227678 Enhancing the Biological Activity of Immobilized Osteopontin Using a Type-1 Collagen Affinity Coating Journal of Biomedical Materials Research. Part A. Jul, 2004 | Pubmed ID: 15174104 Regulation of Cardiovascular Calcification Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology. 2004 | Pubmed ID: 15033154 Adhesive Protein Interactions with Chitosan: Consequences for Valve Endothelial Cell Growth on Tissue-engineering Materials Journal of Biomedical Materials Research. Part A. Nov, 2003 | Pubmed ID: 14566796 Expression and Function of the Integrin Alpha9beta1 in Bovine Aortic Valve Interstitial Cells The Journal of Heart Valve Disease. Sep, 2003 | Pubmed ID: 14565714 Characterization and Analysis of Osteopontin-immobilized Poly(2-hydroxyethyl Methacrylate) Surfaces Journal of Biomedical Materials Research. Part A. Oct, 2003 | Pubmed ID: 14517893 Biomimetic Peptides That Engage Specific Integrin-dependent Signaling Pathways and Bind to Calcium Phosphate Surfaces Journal of Biomedical Materials Research. Part A. Oct, 2003 | Pubmed ID: 14517863 Vascular Calcification: in Vitro Evidence for the Role of Inorganic Phosphate Journal of the American Society of Nephrology : JASN. Sep, 2003 | Pubmed ID: 12939385 Immunohistologic Labeling of Murine Endothelium Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology. Mar-Apr, 2003 | Pubmed ID: 12684163 Osteopontin Inhibits Mineral Deposition and Promotes Regression of Ectopic Calcification The American Journal of Pathology. Dec, 2002 | Pubmed ID: 12466120 Inactivation of the Osteopontin Gene Enhances Vascular Calcification of Matrix Gla Protein-deficient Mice: Evidence for Osteopontin As an Inducible Inhibitor of Vascular Calcification in Vivo The Journal of Experimental Medicine. Oct, 2002 | Pubmed ID: 12391016 Effects of Cyclosporine in Osteopontin Null Mice Kidney International. Jul, 2002 | Pubmed ID: 12081566 Ectopic Calcification: Gathering Hard Facts About Soft Tissue Mineralization The American Journal of Pathology. Mar, 1999 | Pubmed ID: 10079244 NF-kappaB Mediates Alphavbeta3 Integrin-induced Endothelial Cell Survival The Journal of Cell Biology. May, 1998 | Pubmed ID: 9585425 Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells Alexander Blümke1,2, Jessica Simon1, Elizabeth Leber1, Marta Scatena1, Cecilia M. Giachelli1 1Department of Bioengineering, Department of Medicine, University of Washington, 2Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim, Heidelberg University JoVE 66527 Developmental Biology
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells Alexander Blümke1,2, Jessica Simon1, Elizabeth Leber1, Marta Scatena1, Cecilia M. Giachelli1 1Department of Bioengineering, Department of Medicine, University of Washington, 2Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim, Heidelberg University JoVE 66527 Developmental Biology
