David R. Walt Department of Chemistry Tufts University Biography Publications Institution JoVE Articles David R. Walt has not added a biography. If you are David R. Walt and would like to personalize this page please email our Author Liaison for assistance. Publications Plasma IL-6 Changes Correlate to PD-1 Inhibitor Responses in NSCLC Journal for Immunotherapy of Cancer. Oct, 2020 | Pubmed ID: 33020238 Ultrasensitive High-resolution Profiling of Early Seroconversion in Patients with COVID-19 Nature Biomedical Engineering. 12, 2020 | Pubmed ID: 32948854 Ultra-sensitive Serial Profiling of SARS-CoV-2 Antigens and Antibodies in Plasma to Understand Disease Progression in COVID-19 Patients with Severe Disease Clinical Chemistry. Sep, 2020 | Pubmed ID: 32897389 Evaluation of Two Commercial and Two Non-commercial Immunoassays for the Detection of Prior Infection to SARS-CoV-2 MedRxiv : the Preprint Server for Health Sciences. Jun, 2020 | Pubmed ID: 32607518 A Rapid Triage Test for Active Pulmonary Tuberculosis in Adult Patients with Persistent Cough Science Translational Medicine. 10, 2019 | Pubmed ID: 31645455 Protein Detection by Counting Molecules Clinical Chemistry. 06, 2019 | Pubmed ID: 30692115 Impact of Clinical Sample Handling and Processing on Ultra-low Level Measurements of Plasma Cytokines Clinical Biochemistry. Mar, 2019 | Pubmed ID: 30633878 Detection of Amyloid β Oligomers Toward Early Diagnosis of Alzheimer's Disease Analytical Biochemistry. 02, 2019 | Pubmed ID: 30267709 Bottom-up Single-molecule Strategy for Understanding Subunit Function of Tetrameric β-galactosidase Proceedings of the National Academy of Sciences of the United States of America. 08, 2018 | Pubmed ID: 30061400 Single Molecule Arrays for Ultra-sensitive Detection of Rat Cytokines in Serum Journal of Immunological Methods. 01, 2018 | Pubmed ID: 29037955 Development of a Rapid Salivary Proteomic Platform for Oral Feeding Readiness in the Preterm Newborn Frontiers in Pediatrics. 2017 | Pubmed ID: 29312906 Long-Term Measurements of Human Inflammatory Cytokines Reveal Complex Baseline Variations Between Individuals The American Journal of Pathology. Dec, 2017 | Pubmed ID: 28919109 Parkinson's Disease Biomarkers: Perspective from the NINDS Parkinson's Disease Biomarkers Program Biomarkers in Medicine. May, 2017 | Pubmed ID: 28644039 Digital Direct Detection of MicroRNAs Using Single Molecule Arrays Nucleic Acids Research. Aug, 2017 | Pubmed ID: 28637221 Using Next-Generation Sequencing to Explore Genetics and Race in the High School Classroom CBE Life Sciences Education. 2017 | Pubmed ID: 28408407 Single-Molecule Arrays for Protein and Nucleic Acid Analysis Annual Review of Analytical Chemistry (Palo Alto, Calif.). 06, 2017 | Pubmed ID: 28301748 Personal Microbiomes and Next-generation Sequencing for Laboratory-based Education FEMS Microbiology Letters. 12, 2016 | Pubmed ID: 27856569 Using Antigen-antibody Binding Kinetic Parameters to Understand Single-Molecule Array Immunoassay Performance Analytical Chemistry. 12, 2016 | Pubmed ID: 27779850 Incorporation of Slow Off-Rate Modified Aptamers Reagents in Single Molecule Array Assays for Cytokine Detection with Ultrahigh Sensitivity Analytical Chemistry. 09, 2016 | Pubmed ID: 27529794 Protein Counting in Single Cancer Cells Analytical Chemistry. Mar, 2016 | Pubmed ID: 26813414 Fiber-optic Array Using Molecularly Imprinted Microspheres for Antibiotic Analysis Chemical Science. May, 2015 | Pubmed ID: 29142687 Single Molecule Array (Simoa) Assay with Optimal Antibody Pairs for Cytokine Detection in Human Serum Samples The Analyst. Sep, 2015 | Pubmed ID: 26270328 Correlations of Salivary Biomarkers with Clinical Assessments in Patients with Cystic Fibrosis PloS One. 2015 | Pubmed ID: 26258476 Ultra-sensitive Protein Detection Via Single Molecule Arrays Towards Early Stage Cancer Monitoring Scientific Reports. Jun, 2015 | Pubmed ID: 26052106 Ultrasensitive Detection of Ricin Toxin in Multiple Sample Matrixes Using Single-Domain Antibodies Analytical Chemistry. Jul, 2015 | Pubmed ID: 26001138 Salivary Diagnostics Using a Portable Point-of-service Platform: a Review Clinical Therapeutics. Mar, 2015 | Pubmed ID: 25732629 Single-molecule Arrays for Ultrasensitive Detection of Host Immune Response to Dengue Virus Infection Journal of Clinical Microbiology. May, 2015 | Pubmed ID: 25694521 Stoichiometry of the α-complementation Reaction of Escherichia Coli β-galactosidase As Revealed Through Single-molecule Studies Biochemistry. Mar, 2015 | Pubmed ID: 25668156 Genome-wide SNP-genotyping Array to Study the Evolution of the Human Pathogen Vibrio Vulnificus Biotype 3 PloS One. 2014 | Pubmed ID: 25526263 Advancing the Speed, Sensitivity and Accuracy of Biomolecular Detection Using Multi-length-scale Engineering Nature Nanotechnology. Dec, 2014 | Pubmed ID: 25466541 Disease Detection by Ultrasensitive Quantification of Microdosed Synthetic Urinary Biomarkers Journal of the American Chemical Society. Oct, 2014 | Pubmed ID: 25198059 Protein Measurements in Microwells Lab on a Chip. Sep, 2014 | Pubmed ID: 24825470 Observing Single Enzyme Molecules Interconvert Between Activity States Upon Heating PloS One. 2014 | Pubmed ID: 24465972 An Automated Integrated Platform for Rapid and Sensitive Multiplexed Protein Profiling Using Human Saliva Samples Lab on a Chip. Mar, 2014 | Pubmed ID: 24448498 Salivary Inflammatory Mediator Profiling and Correlation to Clinical Disease Markers in Asthma PloS One. 2014 | Pubmed ID: 24409298 Multiplexed Salivary Protein Profiling for Patients with Respiratory Diseases Using Fiber-optic Bundles and Fluorescent Antibody-based Microarrays Analytical Chemistry. Oct, 2013 | Pubmed ID: 23972398 Assessing the Stochastic Intermittency of Single Quantum Dot Luminescence for Robust Quantification of Biomolecules Analytical Chemistry. Jul, 2013 | Pubmed ID: 23631644 Dynamic Microbead Arrays for Biosensing Applications Lab on a Chip. Jun, 2013 | Pubmed ID: 23615790 Optical Methods for Single Molecule Detection and Analysis Analytical Chemistry. Feb, 2013 | Pubmed ID: 23215010 Oil-sealed Femtoliter Fiber-optic Arrays for Single Molecule Analysis Lab on a Chip. Jun, 2012 | Pubmed ID: 22311152 InfoBiology by Printed Arrays of Microorganism Colonies for Timed and On-demand Release of Messages Proceedings of the National Academy of Sciences of the United States of America. Oct, 2011 | Pubmed ID: 21949364 Single Cell Time-resolved Quorum Responses Reveal Dependence on Cell Density and Configuration The Journal of Biological Chemistry. Jun, 2011 | Pubmed ID: 21527637 Lessons Learned from the Introduction of Personalized Genotyping into a Medical School Curriculum Genetics in Medicine : Official Journal of the American College of Medical Genetics. Jan, 2011 | Pubmed ID: 21057320 Visualizing Fluorescence: Using a Homemade Fluorescence "Microscope" to View Latent Fingerprints on Paper Journal of Chemical Education. Oct, 2010 | Pubmed ID: 20852733 Bead-based Optical Fiber Arrays for Artificial Olfaction Current Opinion in Chemical Biology. Dec, 2010 | Pubmed ID: 20655803 Single-molecule Enzyme-linked Immunosorbent Assay Detects Serum Proteins at Subfemtomolar Concentrations Nature Biotechnology. Jun, 2010 | Pubmed ID: 20495550 Note: Toward Multiple Addressable Optical Trapping The Review of Scientific Instruments. Feb, 2010 | Pubmed ID: 20192526 Fibre Optic Microarrays Chemical Society Reviews. Jan, 2010 | Pubmed ID: 20023835 Detection and Classification of Ignitable Liquid Residues Using a Fluorescence-based Vapor-sensitive Microsphere Array Journal of Forensic Sciences. Jan, 2010 | Pubmed ID: 20002259 Ubiquitous Sensors: when Will They Be Here? ACS Nano. Oct, 2009 | Pubmed ID: 19856977 Intelligent Medical Diagnostics Via Molecular Logic Journal of the American Chemical Society. Sep, 2009 | Pubmed ID: 19715272 Chromatically Resolved Optical Microscope (CROMoscope): a Grating-based Instrument for Spectral Imaging Analytical Chemistry. Sep, 2009 | Pubmed ID: 19653690 Design, Implementation, and Field Testing of a Portable Fluorescence-based Vapor Sensor Analytical Chemistry. Jul, 2009 | Pubmed ID: 19563211 Microsphere-based Rolling Circle Amplification Microarray for the Detection of DNA and Proteins in a Single Assay Analytical Chemistry. Jul, 2009 | Pubmed ID: 19548682 Temporally Resolved Fluorescence Spectroscopy of a Microarray-based Vapor Sensing System Analytical Chemistry. Jul, 2009 | Pubmed ID: 19518137 Infochemistry and Infofuses for the Chemical Storage and Transmission of Coded Information Proceedings of the National Academy of Sciences of the United States of America. Jun, 2009 | Pubmed ID: 19470465 Multiplexed Sandwich Immunoassays Using Electrochemiluminescence Imaging Resolved at the Single Bead Level Journal of the American Chemical Society. May, 2009 | Pubmed ID: 19361216 Mechanistic Aspects of Horseradish Peroxidase Elucidated Through Single-molecule Studies Journal of the American Chemical Society. May, 2009 | Pubmed ID: 19338338 DNA Detection on Ultrahigh-density Optical Fiber-based Nanoarrays Biosensors & Bioelectronics. Apr, 2009 | Pubmed ID: 19195871 Fiber-optic Microsphere-based Antibody Array for the Analysis of Inflammatory Cytokines in Saliva Analytical Chemistry. Mar, 2009 | Pubmed ID: 19192965 Multiplexed Spectroscopic Detections Annual Review of Analytical Chemistry (Palo Alto, Calif.). 2008 | Pubmed ID: 20636088 Detection of Single-molecule DNA Hybridization Using Enzymatic Amplification in an Array of Femtoliter-sized Reaction Vessels Journal of the American Chemical Society. Sep, 2008 | Pubmed ID: 18763768 Use of Colorimetric Test Strips for Monitoring the Effect of Hemodialysis on Salivary Nitrite and Uric Acid in Patients with End-stage Renal Disease: a Proof of Principle Clinical Chemistry. Sep, 2008 | Pubmed ID: 18676588 Distinct and Long-lived Activity States of Single Enzyme Molecules Journal of the American Chemical Society. Apr, 2008 | Pubmed ID: 18318491 Very High Density Sensing Arrays Chemical Reviews. Feb, 2008 | Pubmed ID: 18229955 Multianalyte Single-cell Analysis with Multiple Cell Lines Using a Fiber-optic Array Analytical Chemistry. Dec, 2007 | Pubmed ID: 17973503 Stochastic Inhibitor Release and Binding from Single-enzyme Molecules Proceedings of the National Academy of Sciences of the United States of America. Nov, 2007 | Pubmed ID: 17965235 Optical-fiber Bundles The FEBS Journal. Nov, 2007 | Pubmed ID: 17937772 Microsensor Arrays for Saliva Diagnostics Annals of the New York Academy of Sciences. Mar, 2007 | Pubmed ID: 17435144 Fiber-based Single Cell Analysis of Reporter Gene Expression in Yeast Two-hybrid Systems Analytical Biochemistry. Jan, 2007 | Pubmed ID: 17113561 Detecting Oxygen Consumption in the Proximity of Saccharomyces Cerevisiae Cells Using Self-assembled Fluorescent Nanosensors Biotechnology and Bioengineering. Feb, 2007 | Pubmed ID: 16878334 Duplexed Sandwich Immunoassays on a Fiber-optic Microarray Analytica Chimica Acta. Mar, 2006 | Pubmed ID: 17723359 Fiber Optic Array Biosensors BioTechniques. Nov, 2006 | Pubmed ID: 17140107 Fiber-optic Microarray for Simultaneous Detection of Multiple Harmful Algal Bloom Species Applied and Environmental Microbiology. Sep, 2006 | Pubmed ID: 16957189 Digital Readout of Target Binding with Attomole Detection Limits Via Enzyme Amplification in Femtoliter Arrays Journal of the American Chemical Society. May, 2006 | Pubmed ID: 16683771 Microbead Chemical Switches: an Approach to Detection of Reactive Organophosphate Chemical Warfare Agent Vapors Journal of the American Chemical Society. Apr, 2006 | Pubmed ID: 16608338 Digital Concentration Readout of Single Enzyme Molecules Using Femtoliter Arrays and Poisson Statistics Nano Letters. Mar, 2006 | Pubmed ID: 16522055 Fiber-optic Microsphere-based Arrays for Multiplexed Biological Warfare Agent Detection Analytical Chemistry. Feb, 2006 | Pubmed ID: 16478092 Fabrication and Optical Characterization of Imaging Fiber-based Nanoarrays Talanta. Sep, 2005 | Pubmed ID: 18970195 Detecting Biological Warfare Agents Emerging Infectious Diseases. Oct, 2005 | Pubmed ID: 16318712 Extending the Longevity of Fluorescence-based Sensor Arrays Using Adaptive Exposure Analytical Chemistry. Oct, 2005 | Pubmed ID: 16194073 Development of a Microfluidic Platform with an Optical Imaging Microarray Capable of Attomolar Target DNA Detection Analytical Chemistry. Sep, 2005 | Pubmed ID: 16131069 Optical Fiber-based Sensors: Application to Chemical Biology Current Opinion in Chemical Biology. Oct, 2005 | Pubmed ID: 16125439 Monitoring "promiscuous" Drug Effects on Single Cells of Multiple Cell Types Analytical Biochemistry. Oct, 2005 | Pubmed ID: 16099418 Detection of Salmonella Spp. Using Microsphere-based, Fiber-optic DNA Microarrays Analytical Chemistry. Aug, 2005 | Pubmed ID: 16053320 Personalized Exposure Assessment: Promising Approaches for Human Environmental Health Research Environmental Health Perspectives. Jul, 2005 | Pubmed ID: 16002370 Individual Cell Migration Analysis Using Fiber-optic Bundles Analytical and Bioanalytical Chemistry. May, 2005 | Pubmed ID: 15900449 Chemistry. Miniature Analytical Methods for Medical Diagnostics Science (New York, N.Y.). Apr, 2005 | Pubmed ID: 15821081 Array-based Binary Analysis for Bacterial Typing Analytical Chemistry. Jan, 2005 | Pubmed ID: 15623311 Simultaneously Monitoring Gene Expression Kinetics and Genetic Noise in Single Cells by Optical Well Arrays Analytical Chemistry. Nov, 2004 | Pubmed ID: 15516119 Optical Fiber-based Biosensors Analytical and Bioanalytical Chemistry. Aug, 2004 | Pubmed ID: 15221182 Living Bacterial Cell Array for Genotoxin Monitoring Analytical Chemistry. May, 2004 | Pubmed ID: 15144203 Reversible Ratiometric Probe for Quantitative DNA Measurements Analytical Chemistry. Feb, 2004 | Pubmed ID: 14961724 Information Coding in Artificial Olfaction Multisensor Arrays Analytical Chemistry. Aug, 2003 | Pubmed ID: 14632130 Spatially Resolved Electrochemiluminescence on an Array of Electrode Tips Analytical Chemistry. Sep, 2003 | Pubmed ID: 14632040 Combinatorial Decoding: an Approach for Universal DNA Array Fabrication Journal of the American Chemical Society. Nov, 2003 | Pubmed ID: 14599214 Fluorescence-based Fibre Optic Arrays: a Universal Platform for Sensing Chemical Society Reviews. Jul, 2003 | Pubmed ID: 12875026 High-density, Microsphere-based Fiber Optic DNA Microarrays Biosensors & Bioelectronics. May, 2003 | Pubmed ID: 12706560 Optical Imaging Fiber-based Live Bacterial Cell Array Biosensor Analytical Biochemistry. Apr, 2003 | Pubmed ID: 12672418 Enhancing Vapor Sensor Discrimination by Mimicking a Canine Nasal Cavity Flow Environment Journal of the American Chemical Society. Apr, 2003 | Pubmed ID: 12656583 The Use of Optical Fiber Bundles Combined with Electrochemistry for Chemical Imaging Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry. Feb, 2003 | Pubmed ID: 12619418 Imaging Optical Sensor Arrays Current Opinion in Chemical Biology. Oct, 2002 | Pubmed ID: 12413555 Automatic Decoding of Sensor Types Within Randomly Ordered, High-density Optical Sensor Arrays Analytical and Bioanalytical Chemistry. Apr, 2002 | Pubmed ID: 12194041 Optical Imaging Fiber-based Single Live Cell Arrays: a High-density Cell Assay Platform Analytical Chemistry. Jul, 2002 | Pubmed ID: 12141663 Fabrication of an Optoelectrochemical Microring Array Analytical Chemistry. Apr, 2002 | Pubmed ID: 12033265 High-density Fiber-optic Genosensor Microsphere Array Capable of Zeptomole Detection Limits Analytical Chemistry. Apr, 2002 | Pubmed ID: 11985315 Aluminum Surface Corrosion and the Mechanism of Inhibitors Using PH and Metal Ion Selective Imaging Fiber Bundles Analytical Chemistry. Feb, 2002 | Pubmed ID: 11866069 Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage Candice M. Etson1,2, Petar Todorov1, Nooshin Shatery Nejad2, Nirmala Shrestha2, David R. Walt1,3 1Department of Chemistry, Tufts University, 2Department of Physics, Wesleyan University, 3Wyss Institute at Harvard University JoVE 62112 Biochemistry Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles Shuai Nie1, Elena Benito-Peña1,2, Huaibin Zhang1, Yue Wu3, David R. Walt1 1Department of Chemistry, Tufts University, 2Department of Analytical Chemistry, Complutense University (Spain), 3Department of Electrical and Computer Engineering, Tufts University JoVE 50726 Chemistry
Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage Candice M. Etson1,2, Petar Todorov1, Nooshin Shatery Nejad2, Nirmala Shrestha2, David R. Walt1,3 1Department of Chemistry, Tufts University, 2Department of Physics, Wesleyan University, 3Wyss Institute at Harvard University JoVE 62112 Biochemistry
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles Shuai Nie1, Elena Benito-Peña1,2, Huaibin Zhang1, Yue Wu3, David R. Walt1 1Department of Chemistry, Tufts University, 2Department of Analytical Chemistry, Complutense University (Spain), 3Department of Electrical and Computer Engineering, Tufts University JoVE 50726 Chemistry
