Articles by David Bode in JoVE
Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction David Bode1,2, Tim Guthof1, Burkert M. Pieske1,2, Frank R. Heinzel1,2, Felix Hohendanner1,2 1Department of Internal Medicine and Cardiology, Charité University Medicine, 2German Center for Cardiovascular Research (DZHK) Here, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes from a rat model of metabolic syndrome-related heart failure with preserved ejection fraction. A manual regulation of intraluminal pressure of cardiac cavities is implemented to yield functionally intact myocytes suitable for excitation-contraction-coupling studies.
Other articles by David Bode on PubMed
Cellular Mechanisms of Metabolic Syndrome-related Atrial Decompensation in a Rat Model of HFpEF Journal of Molecular and Cellular Cardiology. Feb, 2018 | Pubmed ID: 29289652 Heart failure (HF) with preserved ejection fraction (HFpEF) is present in about 50% of HF patients. Atrial remodeling is common in HFpEF and associated with increased mortality. We postulate that atrial remodeling is associated with atrial dysfunction in vivo related to alterations in cardiomyocyte Calcium (Ca) signaling and remodeling. We examined atrial function in vivo and Ca transients (CaT) (Fluo4-AM, field stim) in atrial cardiomyocytes of ZSF-1 rats without (Ln; lean hypertensive) and with metabolic syndrome (Ob; obese, hypertensive, diabetic) and HFpEF.