Articles by Douglas K. Reilly in JoVE
Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons Douglas K. Reilly1, Daniel E. Lawler2, Dirk R. Albrecht1,2, Jagan Srinivasan1 1Department of Biology and Biotechnology, Worcester Polytechnic Institute, 2Department of Biomedical Engineering, Worcester Polytechnic Institute The use of an adapted "olfactory chip" for the efficient calcium imaging of C. elegans males is described here. Studies of male exposure to glycerol and a pheromone are also shown.
Other articles by Douglas K. Reilly on PubMed
Reproductive Evolution: Pulling the Plug on Selection Current Biology : CB. Oct, 2015 | Pubmed ID: 26485371 Hermaphroditism leads to reduced sexual selection and can result in the retention of deleterious mutations. A new study characterizes one such mutation that results in male-male copulation in nematodes, while also implicating a previously undescribed source of chemical signaling.
Contrasting Responses Within a Single Neuron Class Enable Sex-specific Attraction in Caenorhabditis Elegans Proceedings of the National Academy of Sciences of the United States of America. Mar, 2016 | Pubmed ID: 26903633 Animals find mates and food, and avoid predators, by navigating to regions within a favorable range of available sensory cues. How are these ranges set and recognized? Here we show that male Caenorhabditis elegans exhibit strong concentration preferences for sex-specific small molecule cues secreted by hermaphrodites, and that these preferences emerge from the collective dynamics of a single male-specific class of neurons, the cephalic sensory neurons (CEMs). Within a single worm, CEM responses are dissimilar, not determined by anatomical classification and can be excitatory or inhibitory. Response kinetics vary by concentration, suggesting a mechanism for establishing preferences. CEM responses are enhanced in the absence of synaptic transmission, and worms with only one intact CEM show nonpreferential attraction to all concentrations of ascaroside for which CEM is the primary sensor, suggesting that synaptic modulation of CEM responses is necessary for establishing preferences. A heterogeneous concentration-dependent sensory representation thus appears to allow a single neural class to set behavioral preferences and recognize ranges of sensory cues.