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Neurobiology: The study of the structure, growth, activities, and functions of Neurons and the Nervous system.

An Introduction to Developmental Neurobiology

JoVE 5207

Developmental neuroscience is a field that explores how the nervous system is formed, from early embryonic stages through adulthood. Although it is known that neural progenitor cells follow predictable stages of proliferation, differentiation, migration, and maturation, the mechanisms controlling the progression through each stage are incompletely understood. Studying development is not only important for understanding how complex structures are assembled, but also for characterizing and treating developmental disorders. Since injury repair processes are similar to those that occur in development, this field is also a promising source of insight into when and how nervous system tissues regenerate.This video provides a brief overview of the field of developmental neuroscience, including some key experiments that have advanced our understanding of the mechanisms controlling the formation of early neural tissue and the further specialization of those cells into discrete subsets of neurons. The discussion focuses on prominent questions that developmental biologists are asking and then demonstrates some of the methods that they use to investigate these questions. Finally, applications of the techniques are presented to provide insight into what it means to be a developmental neuroscientist today. The range of experiments demo


 Neuroscience

Generation and On-Demand Initiation of Acute Ictal Activity in Rodent and Human Tissue

1Division of Fundamental Neurobiology, Krembil Research Institute, 2Institute of Medical Science, Faculty of Medicine, University of Toronto, 3Institute of Biomaterials and Biomedical Engineering, University of Toronto, 4Division of Neurosurgery, Department of Surgery, University of Toronto, 5Division of Neurology, Department of Medicine, University of Toronto, 6Department of Physiology, University of Toronto

Video Coming Soon

JoVE 57952


 JoVE In-Press

Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector

1Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences, KU Leuven, 2Division of Nuclear Medicine, Leuven University Hospital - KU Leuven, 3Laboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, 4Leuven Viral Vector Core, KU Leuven

JoVE 53670


 Medicine

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

1Leibniz Institute for Neurobiology (LIN), 2Institute of Experimental Internal Medicine, Medical School, Otto von Guericke University Magdeburg, 3Institute of Pharmacology and Toxicology, Medical School, Otto von Guericke University

JoVE 54992


 Neuroscience

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

1Emmy Noether-Group for Stem Cell Biology, Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg, 2Spemann Graduate School of Biology and Medicine and Faculty of Biology, University of Freiburg, 3School of Life Sciences, Keele University, 4Center for Biological Signaling Studies (BIOSS), University of Freiburg

JoVE 52241


 Neuroscience

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

1Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, 2Center for Brain Injury & Repair, Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, 3Center for Neurotrauma, Neurodegeneration & Restoration, Michael J. Crescenz Veterans Affairs Medical Center, 4School of Biomedical Engineering, Drexel University

JoVE 55609


 Neuroscience

In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons

1Unit on Neural Circuits and Adaptive Behaviors, Genes Cognition and Psychosis Program, National Institute of Mental Health, 2Department of Neuroscience, Brown University - National Institutes of Health Graduate Partnership Program, 3Section on Synaptic Pharmacology, Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, 4Champalimaud Neuroscience Programme, Champalimaud Center for the Unknown

JoVE 50148


 Neuroscience

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

1Department of Neurobiology, F. M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, 2Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute, Wellcome Genome Campus, 3Centre for Molecular Informatics, Department of Chemistry, University of Cambridge, 4Functional Proteomics Group, Chester Beatty Laboratories, Institute of Cancer Research

JoVE 57633


 Genetics

Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System

1Department of Clinical Neurosciences, Arnie Charbonneau Cancer Institute, University of Calgary, 2Department of Cell Biology and Anatomy, Hotchkiss Brain Institute, University of Calgary, 3Department of Oncology, Hotchkiss Brain Institute, University of Calgary

JoVE 57440


 Bioengineering

Tickling, a Technique for Inducing Positive Affect When Handling Rats

1Center for the Study of Animal Well-being, Department of Integrative Physiology and Neuroscience, Washington State University, 2Canadian Council on Animal Care, 3Department of Animal Sciences, Center for Animal Welfare Science, College of Agriculture, Purdue University, 4Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences

JoVE 57190


 Behavior

A Protocol for Measuring Cue Reactivity in a Rat Model of Cocaine Use Disorder

1Center for Addiction Research, University of Texas Medical Branch, 2Institute for Translational Sciences, University of Texas Medical Branch, 3Mitchell Center for Neurodegenerative Diseases, University of Texas Medical Branch, 4Department of Neurology, University of Texas Medical Branch, 5Department of Pathology, University of Texas Medical Branch, 6Department of Pharmacology and Toxicology, University of Texas Medical Branch

JoVE 55864


 Neuroscience

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