Articles by Elias Uhlin

In JoVE (1)

Integreringsfri avledning av humane inducerte pluripotente stamceller ved bruk av Laminin 521 Matrix

Other Publications (1)

Stem Cell Research

Articles by Elias Uhlin in JoVE

Developmental Biology

Integreringsfri avledning av humane inducerte pluripotente stamceller ved bruk av Laminin 521 Matrix Elias Uhlin¹, Ana Marin Navarro^1,2, Harriet Rönnholm¹, Kelly Day¹, Malin Kele¹, Anna Falk¹ ¹Department of Neuroscience, Karolinska Institutet, ²Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet Robust avledning av humant indusert pluripotent stamceller (hiPS) celler ble oppnådd ved å bruke ikke-integrering av Sendai virus (SeV) vektor-mediert omprogrammering av dermale fibroblaster. HiPS-cellevedlikehold og klonal ekspansjon ble utført ved hjelp av xenofri og kjemisk definerte kulturbetingelser med rekombinant human laminin 521 (LN-521) matriks og essensielt E8 (E8) medium.

Other articles by Elias Uhlin on PubMed

Derivation of Human IPS Cell Lines from Monozygotic Twins in Defined and Xeno Free Conditions Stem Cell Research. Jan, 2017 | Pubmed ID: 28395796 Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay.

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