Articles by Eva-Maria Dehne in JoVE
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2 Thomas Hiller1, Viola Röhrs1, Eva-Maria Dehne2, Anke Wagner1,3, Henry Fechner1, Roland Lauster2, Jens Kurreck1 1Department of Applied Biochemistry, Institute of Biotechnology, Berlin University of Technology, 2Department of Medical Biotechnology, Institute of Biotechnology, Berlin University of Technology, 3Department of Bioprocess Engineering, Institute of Biotechnology, Berlin University of Technology The recellularized extracellular matrix of a decellularized rat liver can be used as a humanized, three-dimensional ex vivo model to study the distribution and transgene expression of a virus or viral vector.
Other articles by Eva-Maria Dehne on PubMed
Validation of Bioreactor and Human-on-a-Chip Devices for Chemical Safety Assessment Advances in Experimental Medicine and Biology. 2016 | Pubmed ID: 27671728 Equipment and device qualification and test assay validation in the field of tissue engineered human organs for substance assessment remain formidable tasks with only a few successful examples so far. The hurdles seem to increase with the growing complexity of the biological systems, emulated by the respective models. Controlled single tissue or organ culture in bioreactors improves the organ-specific functions and maintains their phenotypic stability for longer periods of time. The reproducibility attained with bioreactor operations is, per se, an advantage for the validation of safety assessment. Regulatory agencies have gradually altered the validation concept from exhaustive "product" to rigorous and detailed process characterization, valuing reproducibility as a standard for validation. "Human-on-a-chip" technologies applying micro-physiological systems to the in vitro combination of miniaturized human organ equivalents into functional human micro-organisms are nowadays thought to be the most elaborate solution created to date. They target the replacement of the current most complex models-laboratory animals. Therefore, we provide here a road map towards the validation of such "human-on-a-chip" models and qualification of their respective bioreactor and microchip equipment along a path currently used for the respective animal models.