Articles by Hermann Goetz in JoVE
A Volumetric Method for Quantification of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage Axel Neulen*1, Michael Kosterhon*1, Tobias Pantel*1, Stefanie Kirschner2, Hermann Goetz3, Marc A. Brockmann2, Sven R. Kantelhardt1, Serge C. Thal4 1Department of Neurosurgery, Medical Center of the Johannes Gutenberg - University, 2Department of Neuroradiology, Medical Center of the Johannes Gutenberg - University, 3Platform for Biomaterial Research, Medical Center of the Johannes Gutenberg - University, 4Department of Anesthesiology, Medical Center of the Johannes Gutenberg - University The goal of this article is to present a method that allows a 3-dimensional reconstruction of the cerebrovascular tree in mice after micro computed tomography and determination of volumes of entire vessel segments that can be used to quantify cerebral vasospasm in murine models of subarachnoid hemorrhage.
Other articles by Hermann Goetz on PubMed
The Role of Muscular Trauma in the Development of Heterotopic Ossification After Hip Surgery: An Animal-model Study in Rats Injury. Mar, 2016 | Pubmed ID: 26653772 Heterotopic ossification (HO), the formation of bone in soft tissues, is a frequent complication after surgery of the hip and the pelvis. Although the pathophysiological entities responsible for the formation of HO remain largely unclear, muscle trauma is alleged to play a central role in the pathogenic mechanisms underlying HO. However, for this observation, made by many surgeons for decades, no objective evidence has been provided yet.
A New Bone Substitute Developed from 3D-Prints of Polylactide (PLA) Loaded with Collagen I: An In Vitro Study International Journal of Molecular Sciences. Nov, 2017 | Pubmed ID: 29186036 Although a lot of research has been performed, large segmental bone defects caused by trauma, infection, bone tumors or revision surgeries still represent big challenges for trauma surgeons. New and innovative bone substitutes are needed. Three-dimensional (3D) printing is a novel procedure to create 3D porous scaffolds that can be used for bone tissue engineering. In the present study, solid discs as well as porous cage-like 3D prints made of polylactide (PLA) are coated or filled with collagen, respectively, and tested for biocompatibility and endotoxin contamination. Microscopic analyses as well as proliferation assays were performed using various cell types on PLA discs. Stromal-derived factor (SDF-1) release from cages filled with collagen was analyzed and the effect on endothelial cells tested. This study confirms the biocompatibility of PLA and demonstrates an endotoxin contamination clearly below the FDA (Food and Drug Administration) limit. Cells of various cell types (osteoblasts, osteoblast-like cells, fibroblasts and endothelial cells) grow, spread and proliferate on PLA-printed discs. PLA cages loaded with SDF-1 collagen display a steady SDF-1 release, support cell growth of endothelial cells and induce neo-vessel formation. These results demonstrate the potential for PLA scaffolds printed with an inexpensive desktop printer in medical applications, for example, in bone tissue engineering.