Articles by Jay Reddy in JoVE
Noninvasive Assessment of Cardiac Abnormalities in Experimental Autoimmune Myocarditis by Magnetic Resonance Microscopy Imaging in the Mouse Chandirasegaran Massilamany*1, Vahid Khalilzad-Sharghi*2, Arunakumar Gangaplara1, David Steffen1, Shadi F. Othman2, Jay Reddy1 1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, 2Department of Biological Systems Engineering, University of Nebraska-Lincoln This study demonstrates the successful establishment of magnetic resonance microscopy imaging as a non-invasive tool to assess the cardiac abnormalities in mice affected with autoimmune myocarditis. The data indicate that the technique can be used to monitor the disease-progression in live animals.
In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers Chandirasegaran Massilamany1, Arunakumar Gangaplara1, Ting Jia1, Christian Elowsky2, Qingsheng Li3, You Zhou2, Jay Reddy1 1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, 2Center for Biotechnology, University of Nebraska, Lincoln, 3Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln The protocol to detect self-reactive CD4 T cells in brain and heart by direct staining with major histocompatibility complex class II dextramers has been described in this report. For comprehensive analysis, a reliable method to enumerate the frequencies of antigen-specific CD4+ T cells in situ is also devised.