Articles by Jonathan Rick in JoVE
Stem-cell Based Engineered Immunity Against HIV Infection in the Humanized Mouse Model Anjie Zhen1, Valerie Rezek1, Cindy Youn1, Jonathan Rick1, Brianna Lam1, Nelson Chang1, Jerome Zack1, Masakazu Kamata1, Scott Kitchen1 1David Geffen School of Medicine, University of California, Los Angeles This protocol describes the methods in constructing a humanized bone-marrow/liver/thymus mouse model with stem cell-based engineered immunity against HIV infection.
Other articles by Jonathan Rick on PubMed
HIV-specific Immunity Derived From Chimeric Antigen Receptor-engineered Stem Cells Molecular Therapy : the Journal of the American Society of Gene Therapy. Aug, 2015 | Pubmed ID: 26050990 The human immunodeficiency virus (HIV)-specific cytotoxic T lymphocyte (CTL) response is critical in controlling HIV infection. Since the immune response does not eliminate HIV, it would be beneficial to develop ways to enhance the HIV-specific CTL response to allow long-term viral suppression or clearance. Here, we report the use of a protective chimeric antigen receptor (CAR) in a hematopoietic stem/progenitor cell (HSPC)-based approach to engineer HIV immunity. We determined that CAR-modified HSPCs differentiate into functional T cells as well as natural killer (NK) cells in vivo in humanized mice and these cells are resistant to HIV infection and suppress HIV replication. These results strongly suggest that stem cell-based gene therapy with a CAR may be feasible and effective in treating chronic HIV infection and other morbidities.