In JoVE (1)
Other Publications (1)
Articles by Kelda Chia in JoVE
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains Julie Mazzolini1, Kelda Chia1, Dirk Sieger1 1Centre for Discovery Brain Sciences, University of Edinburgh We present a protocol to isolate neurons, macrophages and microglia from larval zebrafish brains under physiological and pathological conditions. Upon isolation, RNA is extracted from these cells to analyze their gene expression profile. This protocol allows for the collection of high-quality RNA for performing downstream analysis like qPCR and transcriptomics.
Other articles by Kelda Chia on PubMed
Tumor Initiating Cells Induce Cxcr4-mediated Infiltration of Pro-tumoral Macrophages into the Brain ELife. Feb, 2018 | Pubmed ID: 29465400 It is now clear that microglia and macrophages are present in brain tumors, but whether or how they affect initiation and development of tumors is not known. Exploiting the advantages of the zebrafish () model, we showed that macrophages and microglia respond immediately upon oncogene activation in the brain. Overexpression of human AKT1 within neural cells of larval zebrafish led to a significant increase in the macrophage and microglia populations. By using a combination of transgenic and mutant zebrafish lines, we showed that this increase was caused by the infiltration of peripheral macrophages into the brain mediated via Sdf1b-Cxcr4b signaling. Intriguingly, confocal live imaging reveals highly dynamic interactions between macrophages/microglia and pre-neoplastic cells, which do not result in phagocytosis of pre-neoplastic cells. Finally, depletion of macrophages and microglia resulted in a significant reduction of oncogenic cell proliferation. Thus, macrophages and microglia show tumor promoting functions already during the earliest stages of the developing tumor microenvironment.