Articles by Kevin D. Quinn in JoVE
Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis Charmion Cruickshank-Quinn1, Kevin D. Quinn1, Roger Powell1, Yanhui Yang1, Michael Armstrong1, Spencer Mahaffey2, Richard Reisdorph1, Nichole Reisdorph1 1Department of Immunology, National Jewish Health, 2Department of Pharmacology, School of Medicine, University of Colorado Denver The reliability of results in metabolomics experiments depends on the effectiveness and reproducibility of the sample preparation. Described is a rigorous and in-depth method that enables extraction of metabolites from biological fluids with the option of subsequently analyzing up to thousands of compounds, or just the compound classes of interest.
Other articles by Kevin D. Quinn on PubMed
Ultra High-mass Resolution Paper Spray by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry International Journal of Analytical Chemistry. 2012 | Pubmed ID: 22606203 Paper Spray Ionization is an atmospheric pressure ionization technique that utilizes an offline electro-osmotic flow to generate ions off a paper medium. This technique can be performed on a Bruker SolariX Fourier transform ion cyclotron resonance mass spectrometer by modifying the existing nanospray source. High-resolution paper spray spectra were obtained for both organic and biological samples to demonstrate the benefit of linking the technique with a high-resolution mass analyzer. Error values in the range 0.23 to 2.14 ppm were obtained for calf lung surfactant extract with broadband mass resolving power (m/Δm(50%)) above 60,000 utilizing an external calibration standard.
Tandem Mass Spectrometry of Bilin Tetrapyrroles by Electrospray Ionization and Collision-induced Dissociation Rapid Communications in Mass Spectrometry : RCM. Aug, 2012 | Pubmed ID: 22777778 Bilins are metabolic products of hosts and bacteria on porphyrins, and are markers of health state and human waste contamination. Although bilin tandem mass spectrometry reports exist, their fragmentation behavior as a function of structure has not been compared, nor has fragmentation been examined as a function of collision energy.