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Articles by Klaus P. Knoch in JoVE
Isolation of Human Islets from Partially Pancreatectomized Patients Gregor Bötticher1, Dorothèe Sturm1, Florian Ehehalt1, Klaus P. Knoch2, Stephan Kersting1, Robert Grützmann1, Gustavo B. Baretton3, Michele Solimena2, Hans D. Saeger1 1Department of GI-, Thorax- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 2Molecular Diabetology, Paul Langerhans Institute Dresden, 3Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden The supply of type 2 diabetic islets for research is insufficient. Here we share our protocol for isolating islets from patients undergoing partial pancreatectomy. This approach represents a unique venue for obtaining islets from type 2 diabetic and clinically matched non-diabetic subjects in adequate numbers for basic and clinical studies.