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Isolation of Human Islets from Partially Pancreatectomized Patients
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JoVE Journal Medicine
Isolation of Human Islets from Partially Pancreatectomized Patients

Isolation of Human Islets from Partially Pancreatectomized Patients

DOI:
10.3791/2962-v

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11:10 min

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July 30, 2011

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, , , , , , , ,
1Department of GI-, Thorax- and Vascular Surgery,University Hospital Carl Gustav Carus, University of Technology Dresden, 2Molecular Diabetology,Paul Langerhans Institute Dresden, 3Department of Pathology,University Hospital Carl Gustav Carus, University of Technology Dresden

Chapters

  • 00:03Title
  • 00:05Introduction
  • 00:54Surgical Procedure
  • 01:35Pathology
  • 07:01Collection and Culture
  • 09:07Conclusion

Summary

Automatic Translation

Automatic Translation

The supply of type 2 diabetic islets for research is insufficient. Here we share our protocol for isolating islets from patients undergoing partial pancreatectomy. This approach represents a unique venue for obtaining islets from type 2 diabetic and clinically matched non-diabetic subjects in adequate numbers for basic and clinical studies.

Tags

Human IsletsIsolationPartially Pancreatectomized PatientsType 2 DiabetesIslets Of Langerhans MalfunctionLimited AvailabilityOrgan DonorsPartial PancreatectomyPancreatic DiseasesConsentLocal Ethics CommitteeSurgical SpecimensDiagnostic Purposes1000 IsletsEnzyme-containing MediaDigestionSurface AreaRicordi Chamber

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