Other Publications (1)
Articles by Kyle McCulloch in JoVE
Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants Yan Zhang1,2, Kyle McCulloch2, Jarema Malicki2 1The Second Teaching Hospital of Jilin University, 2Department of Ophthalmology, Harvard Medical School Lens development involves interactions with other tissues. Several zebrafish eye mutants are characterized by an abnormally small lens size. Here we demonstrate a lens transplantation experiment to determine whether this phenotype is due to intrinsic causes or defective interactions with tissues that surround the lens.
Other articles by Kyle McCulloch on PubMed
Small Molecule Screen for Compounds That Affect Vascular Development in the Zebrafish Retina Mechanisms of Development. May-Jun, 2009 | Pubmed ID: 19445054 Blood vessel formation in the vertebrate eye is a precisely regulated process. In the human retina, both an excess and a deficiency of blood vessels may lead to a loss of vision. To gain insight into the molecular basis of vessel formation in the vertebrate retina and to develop pharmacological means of manipulating this process in a living organism, we further characterized the embryonic zebrafish eye vasculature, and performed a small molecule screen for compounds that affect blood vessel morphogenesis. The screening of approximately 2000 compounds revealed four small molecules that at specific concentrations affect retinal vessel morphology but do not produce obvious changes in trunk vessels, or in the neuronal architecture of the retina. Of these, two induce a pronounced widening of vessel diameter without a substantial loss of vessel number, one compound produces a loss of retinal blood vessels accompanied by a mild increase of their diameter, and finally one other generates a severe loss of retinal vessels. This work demonstrates the utility of zebrafish as a screening tool for small molecules that affect eye vasculature and presents several compounds of potential therapeutic importance.