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Articles by Makoto Kondo in JoVE
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Expansão do sangue periférico humano γδ células T usando zoledronato
Makoto Kondo1,2, Takamichi Izumi1,2, Nao Fujieda1,2, Atsushi Kondo1,2, Takeharu Morishita1,2, Hirokazu Matsushita1, Kazuhiro Kakimi1
1Department of Immunotherapeutics (Medinet), University of Tokyo Hospital, 2MEDINET Co., Ltd
Um método para expandir células T γδ a partir de células mononucleares do sangue periférico (PBMC) é descrito. PBMC células derivadas de T γδ são estimuladas e expandida usando zoledronato e interleucina-2 (IL-2). Expansão em grande escala de células T γδ podem ser aplicadas a imunoterapia celular autóloga de câncer.
Other articles by Makoto Kondo on PubMed
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Clear Cell Myoepithelial Carcinoma of the Breast: a Case Report
The Tohoku Journal of Experimental Medicine.
Jun, 2003 |
Pubmed ID: 12962408 Primary clear cell myoepithelial carcinoma (CCMC) is extremely rare. During the past twenty years, only one CCMC case has been reported. Here, a case of CCMC is reported. A 30-year-old woman became aware of an abnormal mass on the left breast, and quadrantectomy was performed. Microscopically, it consisted of tumor cells that had clear cytoplasm and showed an invasive growth pattern. Immunohistochemically, the tumor was positive for alpha-smooth muscle actin, S-100, partly positive for vimentin and epithelial membranous antigen. From these findings, the tumor was diagnosed as CCMC. Most of the clear cell myoepithelial tumors are probably misclassified in other examples. It is expected that the numbers of CCMC may be increased by routinely performing special stains and immunohistochemical stains, even without ultrastructual study.
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Screening for Symptomatic Metal Sensitivity: a Prospective Study of 92 Patients Undergoing Total Knee Arthroplasty
Biomaterials.
Mar, 2005 |
Pubmed ID: 15369690 Metal sensitivity (MS) reactions to implant metals represent a rare but well-documented complication following total joint arthroplasty (TJA). Although 20-25% of post-TJA patients develop MS, only a few highly susceptible patients (< 1%) exhibit symptoms. Whether surgeons should perform screening for MS is currently a matter of debate. The present study investigated the clinical importance of screening for patients predisposed to symptomatic MS, and the specific metals causing symptomatic MS following total knee arthroplasty (TKA). Between 2000 and 2002, a total of 108 primary TKAs were performed on 92 patients. Preoperatively, all patients underwent modified lymphocyte stimulation test (mLST) to Ni, Co, Cr, and Fe. Of the 92 patients, 24 (26%) displayed positive preoperative responses to at least one metal. Five patients displayed implant metal-related eczema and were all mLST-positive preoperatively, suggesting that screening for symptomatic MS is clinically useful. Two of these underwent revision TKA and thereafter, eczema healed and mLST results changed from positive to negative. All mLST-positive patients were divided into three groups: Group I, patients with eczema; Group II, patients with clear history of MS; and Group III, patients neither eczema nor history of MS. When the type of sensitive metals were compared among the three groups, a significant association between presence of Cr-sensitivity and development of eczema (P < 0.05) was identified. No significant association was observed between other metals and development of eczema or history of MS. This indicates that Cr is a potential candidate metal for causing eczema in our TKA series, and Cr-sensitivity may offer a potential predictor for symptomatic MS. The present study indicates that the surgeons should undertake routine preoperative screening for MS, particularly to Cr.
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Suspected Paclitaxel-induced Pneumonitis
Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association.
2006 |
Pubmed ID: 17235637 We report two patients with advanced gastric cancer with suspected paclitaxel-induced interstitial pneumonitis. Case 1, a 66-year-old man with recurrent gastric cancer, was treated weekly with paclitaxel. After 11 administrations of paclitaxel, he developed a nonproductive cough and dyspnea. Computed tomography (CT) scan showed extensive bilateral areas of ground-glass attenuation. He did not respond to corticosteroid, and died 57 days after the last paclitaxel administration. Case 2, a 61-year-old man with gastric cancer and liver metastasis, was treated with weekly paclitaxel. The liver metastasis was dramatically reduced in size, but he developed a nonproductive cough and dyspnea after 21 administrations of paclitaxel. He did not respond to high-dose corticosteroid, and died 50 days after the last paclitaxel administration. Although it is a rare complication, paclitaxel-induced interstitial pneumonitis needs to be considered in the differential diagnosis when patients treated with paclitaxel present with fever, nonproductive cough, and dyspnea.
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Patellar Impingement Against the Tibial Component After Total Knee Arthroplasty
Clinical Orthopaedics and Related Research.
Nov, 2006 |
Pubmed ID: 16760804 Patella baja developed in seven knees in five patients after posterior stabilized total knee arthroplasties when the patella became impinged against the tibial component. Patellar replacement was performed in four knees, and all patients were able to achieve deep flexion postoperatively. The mean followup was 50 months (range, 24-73 months) for these patients. The mean Insall-Salvati ratio changed from 0.87 (range, 0.70-1) immediately postoperatively to 0.66 (range, 0.55-0.84) at followup. Patellar erosion occurred in three knees without patellar replacements, accompanied by pain and reduced range of flexion. The four knees with patellar replacements showed marked erosion of the patellar component or the patella, but all remained asymptomatic. Achieving deep flexion in addition to patella baja was thought to be a key element. Patellar replacement, joint line preservation, shaving the anterior portion of the tibial component, preventing surgically induced patella baja, and careful radiographic followup should be considered when deep flexion is achieved in a knee with patella baja after a total knee arthroplasty.
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Characterization of Family 17 and Family 28 Carbohydrate-binding Modules from Clostridium Josui Cel5A
Bioscience, Biotechnology, and Biochemistry.
May, 2009 |
Pubmed ID: 19420681 The endoglucanase Cel5A from an anaerobic celluloltyic bacterium, Clostridium josui, contains family 17 CBM (CjCBM17) and family 28 CBM (CjCBM28) in tandem. Individual CjCBM17 and CjCBM28 and tandem CjCBM17/28 were constructed to determine the binding characteristics of CjCBM17/28 and to compare the binding affinity of the three CBMs. CjCBM17/28 bound to non-crystalline cellulose, soluble cellulose derivatives, and oat-spelt xylan, but not to birchwood xylan or starch. The thermodynamic parameters for the binding of the CBMs with cellooligosaccharides were determined by isothermal titration calorimetry. The binding of CjCBM28 to cellotetraose and cellopentaose was enthalpically driven (large negative DeltaH value), while that of CjCBM17 was entropically driven (positive DeltaS value). These two CBMs had different mechanisms for binding to cellooligosaccharides. They showed similar binding constants (K(a)) for cellopentaose, but in the case of insoluble polysaccharides, the K(a) of CjCBM17/28 was approximately 3-7 times higher than that of individual CBMs.
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The Nosocomial Transmission of Helicobacter Cinaedi Infections in Immunocompromised Patients
Internal Medicine (Tokyo, Japan).
2010 |
Pubmed ID: 20720350 BACKGROUND: We encountered 15 cases of Helicobacter cinaedi (H. cinaedi) infection between March and July 2008. PATIENT, METHOD, AND RESULT: The underlying diseases were hematological malignancies in a majority of cases, many of which received chemotherapy. All patients had a fever. The fever was followed by cellulitis in three, a skin rash in six, pain in the lower limbs in three, and diarrhea in three cases. We analyzed the bacterial 23S rRNA genes. The fifteen strains were divided according to base sequence into Groups A, B, and C, respectively. All four cases in Group A were women and all ten in Group C were men, indicating that the gender of the patient corresponded precisely to the genotypes of the separated bacilli in these two groups. These findings also suggested the strong possibility of nosocomial spread. CONCLUSION: It is highly likely that H. cinaedi infections have been overlooked due to the difficulties encountered in culturing the bacterium. The possibility of septicemia caused by H. cinaedi should be suspected especially in immunocompromised patients such as those undergoing chemotherapy, with symptoms such as fever, rash, arthritis, cellulitis, leg pain, and other systemic or local symptoms.
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[Remission Induced by Dose-reduction of Immunosuppressants Alone in a Patient with Post-transplant Lymphoproliferative Disorder of Central Nervous System Origin]
[RinshÅ Ketsueki] The Japanese Journal of Clinical Hematology.
May, 2011 |
Pubmed ID: 21646772 A 43-year-old male renal transplant recipient, who received a living related renal transplant 7 years ago and had been maintained with tacrolimus, mycophenolate mofetil (MMF), and prednisolone, was admitted to our hospital complaining of headache and nausea. MRI showed a large mass in the right hemisphere with ring-enhancement indicating brain abscess, tumor or lymphoma. Open biopsy was performed and pathological examination demonstrated diffuse proliferation of polymorphic cells, positive for CD20, bcl-2, EBER, and LMP-1. Based on these findings, primary central nervous system post-transplant lymphoproliferative disorder (PCNS-PTLD) was diagnosed. MMF was discontinued and tacrolimus was tapered. After 2 weeks, MRI showed regression of the tumor size and after 9 months, the tumor had disappeared. Though many reports have shown the severity of PCNS-PTLD, and recommend aggressive treatments such as chemotherapy and/or radiotherapy, our case shows that reduction of immunosuppressant alone with close observation could be a choice of treatment.
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Prevalence of Computed Tomographic Angiography-verified High-risk Plaques and Significant Luminal Stenosis in Patients with Zero Coronary Calcium Score
International Journal of Cardiology.
Mar, 2011 |
Pubmed ID: 21420188 BACKGROUND: Some patients were detected with coronary artery disease even if the coronary artery calcium score was (CACS)=0. We evaluated the prevalence and predictor of significant stenosis and computed tomography (CT) based vulnerable plaque (CTVP) for patients with CACS=0. METHODS: Subjects were 2160 patients (M/F=1110/1050, 64.7±11.6years) who underwent measurement of calcium score and CT coronary angiography. As for CACS=0 group, age, gender, coronary risk factor (family history (FH), hypertension (HT), hyperlipidemia (HL), diabetes (DM), and smoking), body mass index, history of cerebral infarction, the presence of chest symptom, and abnormal rest ECG findings were investigated as predictors for significant stenosis and CTVP by multivariate analysis using logistic regression analysis. RESULTS: Out of 2160 patients, 1141 (52.8%, M/F=655/486, 68.4±9.8years) were of CACS>0 and 1019 (47.2%, M/F=455/564, 60.5±12.0years) were of CACS=0. In the CACS=0 group, 24 patients (2.4%) were found with significant stenosis and 47 (4.6%) with 2FPP. In 104 patients with spotty calcification (10.2%), 10 (9.6%) out of these 104 had significant stenosis and also had CTVP. Multivariate analysis using logistic regression analysis revealed significant predictor for significant stenosis to be only male (Odds ratio (OR): 3.075, 95%CI 1.166-8.109, p=0.0232) and significant predictor for CTVP to be age (OR: 1.032, 95%CI 1.001-1.063, p=0.0437) and male (OR: 2.386, 95%CI 1.193-4.775, p=0.0140). CONCLUSIONS: The present study suggests that the presence of CTVP must be noted, when patients are male and elderly even if CACS=0 and the presence of spotty calcification increases the prevalence of significant stenosis and CTVP in patients with CACS=0.
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Preparation of Keratinocyte Culture Medium for the Clinical Applications of Regenerative Medicine
Journal of Tissue Engineering and Regenerative Medicine.
Apr, 2011 |
Pubmed ID: 20740688 Keratinocyte culture medium (KCM) has been used for the in vitro culture of keratinocytes and other types of epithelial cells, and the medium includes various ingredients. In this study, two modified KCMs were prepared. In the first, insulin, hydrocortisone and antibiotics that are normally included in KCM were replaced with clinically approved pharmaceutical agents, except transferrin and selenium; in the second, cholera toxin (CT) was replaced by L-isoproterenol (ISO). The modified KCMs were then compared to conventional KCM containing laboratory-grade reagents. Induced cell colony formations of canine oral mucosal epithelial cells cultured in both modified KCMs were found to be nearly equivalent to that in the control KCM, and there was no significant difference between the effect of CT and ISO. Canine oral mucosal cells proliferated to confluence in all three KCM formulations, with or without the use of 3T3 feeder layers. Cultured epithelial cells were harvested from temperature-responsive culture surfaces as an intact cell sheet, and the immunohistochemical analysis of the sheets showed that p63 and cytokeratin were expressed in the epithelial cell sheets cultured in all KCMs. Eventually, in the modified KCM formula, fetal bovine serum was replaced by autologous human serum, and the formula was found to be able to fabricate human oral mucosal epithelial cell sheets. These results indicated that the modified KCM was equally efficient as conventional KCM in the fabrication of transplantable stratified epithelial cell sheets.
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