Other Publications (1)
Articles by Nao Fujieda in JoVE
Expansão do sangue periférico humano γδ células T usando zoledronato Makoto Kondo1,2, Takamichi Izumi1,2, Nao Fujieda1,2, Atsushi Kondo1,2, Takeharu Morishita1,2, Hirokazu Matsushita1, Kazuhiro Kakimi1 1Department of Immunotherapeutics (Medinet), University of Tokyo Hospital, 2MEDINET Co., Ltd Um método para expandir células T γδ a partir de células mononucleares do sangue periférico (PBMC) é descrito. PBMC células derivadas de T γδ são estimuladas e expandida usando zoledronato e interleucina-2 (IL-2). Expansão em grande escala de células T γδ podem ser aplicadas a imunoterapia celular autóloga de câncer.
Other articles by Nao Fujieda on PubMed
Amino Acid Substitution at Position 464 in the Haemagglutinin-neuraminidase Protein of a Mumps Virus Urabe Strain Enhanced the Virus Growth in Neuroblastoma SH-SY5Y Cells Vaccine. Oct, 2009 | Pubmed ID: 19712764 Mumps virus (MuV) infects various organs including central nervous system (CNS). However, the molecular basis of the neural cell specificity of MuV is not well understood. We found that the Hoshino vaccine strain rescued from cDNA replicated moderately in neuroblastoma SH-SY5Y cell line, while an Urabe strain (Ur89-250) isolated from a post-vaccination aseptic meningitis case replicated efficiently in the same cells. In order to examine the contribution of individual genes of Ur89-250 to the growth in SH-SY5Y cells, recombinant Hoshino vaccine strains in which each gene(s) was replaced with corresponding gene(s) of Ur89-250 were generated. A recombinant virus possessing the small hydrophobic and haemagglutinin-neuraminidase (HN) genes of Ur89-250 grew as efficiently in SH-SY5Y cells as Ur89-250. Further analysis indicated that an amino acid substitution at position 464 in the HN protein was most important for efficient growth. Thus, single amino acid substitution in the HN protein could affect neural cell specificity of mumps virus.