Other Publications (0)
Articles by Matthew Rea in JoVE
Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm Matthew Rea1, Ming Chen1, Shan Luan1, Drutdaman Bhangu1, Max Braud1, Wenyan Xiao1 1Department of Biology, Saint Louis University Imprinting is a phenomenon in plant and mammal reproduction. DNA methylation plays an important role in mechanisms of imprinting. Isolating endosperm and determining methylation status of imprinted genes in Arabidopsis can be difficult. In this protocol, we describe how to isolate endosperm and determine methylation by bisulfite sequencing.