In JoVE (3)
Other Publications (1)
Articles by Mikhail Yuryev in JoVE
Acute Brain Trauma in Mice Followed By Longitudinal Two-photon Imaging Mikhail Paveliev1, Mikhail Kislin1, Dmitry Molotkov1, Mikhail Yuryev1, Heikki Rauvala1, Leonard Khiroug1 1Neuroscience Center, University of Helsinki Acute brain trauma is a severe injury that has no adequate treatment to date. Multiphoton microscopy allows studying longitudinally the process of acute brain trauma development and probing therapeutical strategies in rodents. Two models of acute brain trauma studied with in vivo two-photon imaging of brain are demonstrated in this protocol.
Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents Mikhail Kislin1, Ekaterina Mugantseva1, Dmitry Molotkov1, Natalia Kulesskaya1, Stanislav Khirug1, Ilya Kirilkin2, Evgeny Pryazhnikov1,2, Julia Kolikova2, Dmytro Toptunov2, Mikhail Yuryev1, Rashid Giniatullin3, Vootele Voikar4, Claudio Rivera1, Heikki Rauvala1, Leonard Khiroug1 1Neuroscience Center, University of Helsinki, 2Neurotar LTD, 3A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, 4Laboratory Animal Center, University of Helsinki This method creates a tangible, familiar environment for the mouse to navigate and explore during microscopic imaging or single-cell electrophysiological recordings, which require firm fixation of the animal’s head.
Other articles by Mikhail Yuryev on PubMed
Dynamic Longitudinal Investigation of Individual Nerve Endings in the Skin of Anesthetized Mice Using in Vivo Two-photon Microscopy Journal of Biomedical Optics. Apr, 2012 | Pubmed ID: 22559685 Visualization of individual cutaneous nerve endings has previously relied on laborious procedures of tissue excision, fixation, sectioning and staining for light or electron microscopy. We present a method for non-invasive, longitudinal two-photon microscopy of single nerve endings within the skin of anesthetized transgenic mice. Besides excellent signal-to-background ratio and nanometer-scale spatial resolution, this method offers time-lapse "movies" of pathophysiological changes in nerve fine structure over minutes, hours, days or weeks. Structure of keratinocytes and dermal matrix is visualized simultaneously with nerve endings, providing clear landmarks for longitudinal analysis. We further demonstrate feasibility of dissecting individual nerve fibers with infra-red laser and monitoring their degradation and regeneration. In summary, our excision-free optical biopsy technique is ideal for longitudinal microscopic analysis of animal skin and skin innervations in vivo and can be applied widely in preclinical models of chronic pain, allergies, skin cancers and a variety of dermatological disorders.