Articles by Morito Sakuma in JoVE
Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering Morito Sakuma1, Yoshikazu Kumashiro2, Masamichi Nakayama2, Nobuyuki Tanaka2, Yuji Haraguchi2, Kazuo Umemura1, Tatsuya Shimizu2, Masayuki Yamato2, Teruo Okano2 1Department of Physics, Tokyo University of Science, 2Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University Nanoscaled sea-island surfaces composed of thermoresponsive block copolymers were fabricated by the Langmuir-Schaefer method for controlling spontaneous cell adhesion and detachment. Both the preparation of the surface and the adhesion and detachment of cells on the surface were visualized.
Other articles by Morito Sakuma on PubMed
Control of Cell Adhesion and Detachment on Langmuir-Schaefer Surface Composed of Dodecyl-terminated Thermo-responsive Polymers Journal of Biomaterials Science. Polymer Edition. 2014 | Pubmed ID: 24328952 This study used Langmuir-Schaefer (LS) method to produce thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) modified surface. Dodecyl terminated-PIPAAm (PIPAAm-C12) was synthesized by reversible addition-fragmentation chain transfer radical polymerization. PIPAAm-C12 was dropped on an air-water interface and formed Langmuir film by compressing. A surface pressure measurement revealed that PIPAAm-C12 was floated and Langmuir films were formed on the interface. And the Langmuir film was transferred on a hydrophobic substrate to produce PIPAAm-C12 transferred surface (PIPAAm-LS surface). In the results of atomic force microscope, attenuated total reflection Fourier transform infrared spectroscope, and X-ray photoelectron spectroscope measurement, the transference of Langmuir films was demonstrated and densities could be precisely controlled. Ellipsometric measurements of PIPAAm-LS surfaces showed that the thicknesses of the surfaces were less than 10 nm. Cell adhesion and detachment were observed on the PIPAAm-LS surfaces. The amount of adhered cells on all LS surfaces was found to be similar on the control hydrophobic substrate at 37 °C. In regard to cell detachment, adhering cells rapidly detached themselves with higher densities and shorter PIPAAm-C12 molecules. In this method, the effect of densities and molecular weights on cell adhesion and detachment were observed. Our method should be proved novel insights for investigating cell adhesion and detachment on thermo-responsive surfaces.
Thermoresponsive Nanostructured Surfaces Generated by the Langmuir-Schaefer Method Are Suitable for Cell Sheet Fabrication Biomacromolecules. Nov, 2014 | Pubmed ID: 25290209 Thermoresponsive poly(N-isopropylacrylamide) (PIPAAm)-immobilized surfaces for controlling cell adhesion and detachment were fabricated by the Langmuir-Schaefer method. Block copolymers composed of polystyrene and PIPAAm (St-IPAAms) having various chain lengths and compositions were synthesized by reversible addition-fragmentation chain transfer radical polymerization. The St-IPAAm Langmuir film at an air-water interface was horizontally transferred onto a hydrophobically modified glass substrate while regulating its density. Atomic force microscopy images clearly visualized nanoscaled sea-island structures on the surface. By adjusting both the composition of St-IPAAms and the density of immobilized PIPAAms, a series of thermoresponsive surfaces was prepared to control the strength, rate, and quality of cell adhesion and detachment through changes in temperature across the lower critical solution temperature range of PIPAAm molecules. In addition, a two-dimensional cell structure (cell sheet) was more rapidly recovered on the optimized surfaces than on conventional PIPAAm surfaces. These unique PIPAAm surfaces are suggested to be useful for controlling the strength of cell adhesion and detachment.