Otto Kučera Department of Physiology Charles University, Faculty of Medicine in Hradec Kralove Biography Publications Institution JoVE Articles Otto Kučera Otto Kučera is an Associate Professor at the Department of Physiology, Charles University, Faculty of Medicine in Hradec Kralove. He received an M.D. with honours degree (2001) and a Ph.D. in Physiology and pathological physiology (2005) from the same faculty.Since 2001, he has joined a team of Experimental hepatology. In addition to his teaching duties in Physiology, Dr Kučera is an experimental scientist who has studied mouse and rat models of non-alcoholic fatty liver disease and hepatotoxic action of model hepatotoxins in vivo and in vitro. An important component of his research program is focused on isolation of rat and mouse hepatocytes and their cultivation in primary cultures. Publications Western Diet Decreases the Liver Mitochondrial Oxidative Flux of Succinate: Insight from a Murine NAFLD Model International Journal of Molecular Sciences. Jun, 2021 | Pubmed ID: 34199098 The Connection Between MicroRNAs from Visceral Adipose Tissue and Non-Alcoholic Fatty Liver Disease Acta Medica (Hradec Kralove). Month, 2021 | Pubmed ID: 33855952 Comparison of Two Anti-diabetic Monoestolides Regarding Effects on Intact Murine Liver Tissue Archives of Physiology and Biochemistry. Mar, 2020 | Pubmed ID: 32208934 Dose-dependent Regulation of Mitochondrial Function and Cell Death Pathway by Sorafenib in Liver Cancer Cells Biochemical Pharmacology. 06, 2020 | Pubmed ID: 32156660 Adaptation of Mitochondrial Substrate Flux in a Mouse Model of Nonalcoholic Fatty Liver Disease International Journal of Molecular Sciences. Feb, 2020 | Pubmed ID: 32046101 Effect of Glucagon-like Peptide-1 Analogue Liraglutide on Primary Cultures of Rat Hepatocytes Isolated from Lean and Steatotic Livers General Physiology and Biophysics. Jul, 2019 | Pubmed ID: 31241045 Stilbene Compound Trans-3,4,5,4´-tetramethoxystilbene, a Potential Anticancer Drug, Regulates Constitutive Androstane Receptor (Car) Target Genes, but Does Not Possess Proliferative Activity in Mouse Liver Toxicology Letters. Oct, 2019 | Pubmed ID: 31170421 Acetaminophen Toxicity in Rat and Mouse Hepatocytes in Vitro Drug and Chemical Toxicology. Oct, 2017 | Pubmed ID: 27960556 Effects of Epigallocatechin Gallate on Tert-Butyl Hydroperoxide-Induced Mitochondrial Dysfunction in Rat Liver Mitochondria and Hepatocytes Oxidative Medicine and Cellular Longevity. Month, 2016 | Pubmed ID: 28074116 Impaired Mitochondrial Functions Contribute to 3-bromopyruvate Toxicity in Primary Rat and Mouse Hepatocytes Journal of Bioenergetics and Biomembranes. 08, 2016 | Pubmed ID: 27530389 Does Simple Steatosis Affect Liver Regeneration After Partial Hepatectomy in Rats? Acta Medica (Hradec Kralove). Month, 2016 | Pubmed ID: 27526303 The Effect of D-galactosamine on Lean and Steatotic Rat Hepatocytes in Primary Culture Physiological Research. Month, 2015 | Pubmed ID: 26674289 Metformin Prevents Ischemia Reperfusion-induced Oxidative Stress in the Fatty Liver by Attenuation of Reactive Oxygen Species Formation American Journal of Physiology. Gastrointestinal and Liver Physiology. Jul, 2015 | Pubmed ID: 26045616 In Vitro Toxicity of Epigallocatechin Gallate in Rat Liver Mitochondria and Hepatocytes Oxidative Medicine and Cellular Longevity. Month, 2015 | Pubmed ID: 25918582 Experimental Models of Non-alcoholic Fatty Liver Disease in Rats World Journal of Gastroenterology. Jul, 2014 | Pubmed ID: 25024595 Antioxidative Effect of Epigallocatechin Gallate Against D-galactosamine-induced Injury in Primary Culture of Rat Hepatocytes Acta Medica (Hradec Kralove). Month, 2014 | Pubmed ID: 25006656 The Effect of Epigallocatechin Gallate on Hepatocytes Isolated from Normal and Partially Hepatectomized Rats Canadian Journal of Physiology and Pharmacology. Jun, 2014 | Pubmed ID: 24853265 The Effect of Tert-butyl Hydroperoxide-induced Oxidative Stress on Lean and Steatotic Rat Hepatocytes in Vitro Oxidative Medicine and Cellular Longevity. Month, 2014 | Pubmed ID: 24847414 Epigallocatechin Gallate Does Not Accelerate the Early Phase of Liver Regeneration After Partial Hepatectomy in Rats Digestive Diseases and Sciences. May, 2014 | Pubmed ID: 24318805 Cholestatic Effect of Epigallocatechin Gallate in Rats is Mediated Via Decreased Expression of Mrp2 Toxicology. Jan, 2013 | Pubmed ID: 23146761 Steatotic Rat Hepatocytes in Primary Culture Are More Susceptible to the Acute Toxic Effect of Acetaminophen Physiological Research / Academia Scientiarum Bohemoslovaca. Month, 2012 | Pubmed ID: 23130907 Susceptibility of Rat Non-alcoholic Fatty Liver to the Acute Toxic Effect of Acetaminophen Journal of Gastroenterology and Hepatology. Feb, 2012 | Pubmed ID: 21649732 Deteriorating Effect of Fluvastatin on the Cholestatic Liver Injury Induced by Bile Duct Ligation in Rats General Physiology and Biophysics. Mar, 2011 | Pubmed ID: 21460414 Is Rat Liver Affected by Non-alcoholic Steatosis More Susceptible to the Acute Toxic Effect of Thioacetamide? International Journal of Experimental Pathology. Aug, 2011 | Pubmed ID: 21410800 The Effect of Rat Strain, Diet Composition and Feeding Period on the Development of a Nutritional Model of Non-alcoholic Fatty Liver Disease in Rats Physiological Research / Academia Scientiarum Bohemoslovaca. Month, 2011 | Pubmed ID: 21114362 The Toxic Effect of Thioacetamide on Rat Liver in Vitro Toxicology in Vitro : an International Journal Published in Association with BIBRA. Dec, 2010 | Pubmed ID: 20600801 Effect of S-adenosylmethionine on Liver Regeneration Induced by Partial Hepatectomy General Physiology and Biophysics. Mar, 2010 | Pubmed ID: 20371883 Up-regulation of Renal Mdr1 and Mrp2 Transporters During Amiodarone Pretreatment in Rats Pharmacological Research : the Official Journal of the Italian Pharmacological Society. Feb, 2010 | Pubmed ID: 19703566 Mechanisms Participating in Oxidative Damage of Isolated Rat Hepatocytes Archives of Toxicology. Apr, 2009 | Pubmed ID: 19020865 S-adenosylmethionine Exerts a Protective Effect Against Thioacetamide-induced Injury in Primary Cultures of Rat Hepatocytes Alternatives to Laboratory Animals : ATLA. Jun, 2007 | Pubmed ID: 17650956 Evaluation of Mitochondrial Function in Isolated Rat Hepatocytes and Mitochondria During Oxidative Stress Alternatives to Laboratory Animals : ATLA. Jun, 2007 | Pubmed ID: 17650955 [Model Systems for Study of Toxic Injury of Hepatocytes in Vitro] Ceskoslovenska Fysiologie. Month, 2006 | Pubmed ID: 17685015 The Model of D-galactosamine-induced Injury of Rat Hepatocytes in Primary Culture Acta Medica (Hradec Kralove). Month, 2006 | Pubmed ID: 16696445 Protective Effect of S-adenosylmethionine Against Galactosamine-induced Injury of Rat Hepatocytes in Primary Culture Physiological Research. Month, 2006 | Pubmed ID: 16343037 Protective Effect of S-adenosylmethionine on Cellular and Mitochondrial Membranes of Rat Hepatocytes Against Tert-butylhydroperoxide-induced Injury in Primary Culture Chemico-biological Interactions. Sep, 2005 | Pubmed ID: 16098496 Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells Moustafa Elkalaf1,2, Karolína Vaněčková1,2, Pavla Staňková1, Zuzana Červinková1, Jan Polák*2,3, Otto Kučera*1 1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady JoVE 62902 Biology
Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells Moustafa Elkalaf1,2, Karolína Vaněčková1,2, Pavla Staňková1, Zuzana Červinková1, Jan Polák*2,3, Otto Kučera*1 1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady JoVE 62902 Biology
