Articles by Pappanaicken Kumaresan in JoVE
Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System Pappanaicken Kumaresan*1, Mathew Figliola*1, Judy S. Moyes1, M. Helen Huls1, Priti Tewari1, Elizabeth J. Shpall2, Richard Champlin2, Laurence J.N. Cooper1 1Division of Pediatrics, U.T. MD Anderson Cancer Center, 2Stem Cell Transplantation and Cellular Therapy, U.T. MD Anderson Cancer Center The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.
Other articles by Pappanaicken Kumaresan on PubMed
Design, Synthesis, and Application of OB2C Combinatorial Peptide and Peptidomimetic Libraries Methods in Molecular Biology (Clifton, N.J.). 2015 | Pubmed ID: 25616322 The "one-bead two-compound" (OB2C) combinatorial library is constructed on topologically segregated trifunctional bilayer beads such that each bead has a fixed cell-capturing ligand and a random library compound co-displayed on its surface and a chemical coding tag (bar code) inside the bead. An OB2C library containing thousands to millions of compounds can be synthesized and screened concurrently within a short period of time. When live cells are incubated with such OB2C libraries, every bead will be coated with a monolayer of cells. The cell membranes of the captured cells facing the bead surface are exposed to the library compounds tethered to each bead. A specific biochemical or cellular response can be detected with an appropriate reporter system. The OB2C method enables investigators to rapidly discover synthetic molecules that not only interact with cell-surface receptors but can also stimulate or inhibit downstream cell signaling. To demonstrate this powerful method, one OB2C peptide library and two OB2C peptidomimetic libraries were synthesized and screened against Molt-4 lymphoma cells to discover "death ligands." Apoptosis of the bead-bound cells was detected with immunocytochemistry using horseradish peroxidase (HRP)-conjugated anti-cleaved caspase-3 antibody and 3,3'-diaminobenzidine as a substrate. Two novel synthetic "death ligands" against Molt-4 cells were discovered using this OB2C library approach.
Genetic Engineering of T Cells to Target HERV-K, an Ancient Retrovirus on Melanoma Clinical Cancer Research : an Official Journal of the American Association for Cancer Research. Jul, 2015 | Pubmed ID: 25829402 The human endogenous retrovirus (HERV-K) envelope (env) protein is a tumor-associated antigen (TAA) expressed on melanoma but not normal cells. This study was designed to engineer a chimeric antigen receptor (CAR) on T-cell surface, such that they target tumors in advanced stages of melanoma.