In JoVE (1)
Other Publications (0)
Articles by Phillip Necaise in JoVE
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks George Merz1, Valerie Schwenk1, Ruchit Shah2, Carolyn Salafia1,2, Phillip Necaise2, Michael Joyce1, Tom Villani3, Michael Johnson3, Nick Crider3 1The Institute for Basic Research, The New York State Office for People with Developmental Disabilities, 2Placental Analytics LLC, 3Visikol Inc. This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm3.