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Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
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Developmental Biology
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JoVE Journal Developmental Biology
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

DOI:
10.3791/57099-v

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09:33 min

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March 29, 2018

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, , , , , , , ,
1The Institute for Basic Research,The New York State Office for People with Developmental Disabilities, 2Placental Analytics LLC, 3Visikol Inc.

Chapters

  • 00:04Title
  • 01:17Villous Tree Dissection and Immunofluorescent Staining
  • 03:16Clarification of the Tissue
  • 04:07Mounting for Microscopy and Confocal Microscopy
  • 06:04Reversing the Clearing
  • 06:33Results: Use of Confocal Microscopy, Immunostaining, and Reverse Clearing to Analyze the Vascularization of Placental Network
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm3.

Tags

3D RenderingImmunolabeledClarifiedPlacental Villous Vascular NetworksVascular AnalysisImmunolabelingCD31CK7Fluorescent LabelingMicroscopyPlacental Growth RestrictionPreterm Birth

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