Journal
/
Developmental Biology
/
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

DOI:
10.3791/57099-v

•

09:33 min

•

March 29, 2018

•

, , , , , , , ,
1The Institute for Basic Research,The New York State Office for People with Developmental Disabilities, 2Placental Analytics LLC, 3Visikol Inc.

Chapters

  • 00:04Title
  • 01:17Villous Tree Dissection and Immunofluorescent Staining
  • 03:16Clarification of the Tissue
  • 04:07Mounting for Microscopy and Confocal Microscopy
  • 06:04Reversing the Clearing
  • 06:33Results: Use of Confocal Microscopy, Immunostaining, and Reverse Clearing to Analyze the Vascularization of Placental Network
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm3.

Tags

Keywords: 3D RenderingImmunolabeledClarifiedPlacental Villous Vascular NetworksVascular AnalysisImmunolabelingCD31CK7Fluorescent LabelingMicroscopyPlacental Growth RestrictionPreterm Birth

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57099?article_type=v

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
check_url/57099?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/57099?article_type=v

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
check_url/57099?article_type=v

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
check_url/57099?article_type=v

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin
check_url/57099?article_type=v

A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse
check_url/57099?article_type=v

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs
check_url/57099?article_type=v

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
check_url/57099?article_type=v

High Frequency Ultrasound for the Analysis of Fetal and Placental Development In Vivo

Read Article