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Articles by René van Lien in JoVE
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Mess-Cardiac Autonomic Nervous System (ANS) Aktivität bei Kindern
Aimée E. van Dijk*1,2, René van Lien*3,4, Manon van Eijsden2,5, Reinoud J. B. J. Gemke6, Tanja G. M. Vrijkotte1, Eco J. de Geus3,4
1Department of Public Health, Academic Medical Center - University of Amsterdam, 2Department of Epidemiology, Documentation and Health Promotion, Public Health Service of Amsterdam (GGD), 3Department of Biological Psychology, VU University, 4EMGO+ Institute, VU University Medical Center, 5Institute of Health Sciences, VU University, 6Department of Pediatrics, VU University Medical Center
Messung der Aktivität des autonomen Nervensystems in der Regel beschränkt die Forscher und Teilnehmer an das Labor, die eine einschüchternde Umwelt für Kinder vorsehen. Die VU University Ambulante Monitoring System (VU-AMS)-Gerät aufzeichnen können kardiale autonome Kontrolle in jeder Einstellung. Die VU-AMS als sehr zugänglich Testen bei Kindern.
Other articles by René van Lien on PubMed
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Tailoring the Coupling Between Localized and Propagating Surface Plasmons: Realizing Fano-like Interference and High-performance Sensor
Optics Express.
Apr, 2013 |
Pubmed ID: 23609734 Surface plasmon modes originated from various metallic nanostructures possess unique features of strong nanoscale light confinement and enhancement with tunable energy, which make them attractive and promising for a variety of applications such as sensing, solar cell, and lasing. Here, we have investigated the interaction between localized and propagating surface plasmons in a structure consisting of a gold nanobar array and a thick gold film, separated by a silica dielectric spacer layer. It is found that the reflection spectrum of the designed plasmonic structure can be readily tailored by changing the gold nanobar size, array period and the spacer layer thickness. Moreover, the strong coupling between the localized and propagating modes can result in an anticrossing behavior and even induce a Fano-like asymmetric lineshape. Importantly, the sensitivity and the figure of merit (FoM) of this plasmonic system can reach as high as 936 nm/RIU and 112, respectively. Our study offers a new, simple, efficient and controllable way to design the plasmonic systems with desired modes coupling and spectral lineshapes for different applications.
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Controllable Synthesis of Recyclable Core-shell γ-Fe2O3@SnO2 Hollow Nanoparticles with Enhanced Photocatalytic and Gas Sensing Properties
Physical Chemistry Chemical Physics : PCCP.
Apr, 2013 |
Pubmed ID: 23612776 Composite materials containing different components with well-defined structures may cooperatively enhance their performance and extend their applications. In this work, core-shell γ-Fe2O3@SnO2 hollow nanoparticles (NPs) were synthesized by a low-cost and environmentally friendly seed-mediated hydrothermal method. Firstly, the γ-Fe2O3 hollow NPs were synthesized by a template-free method. Then they were used as the cores for the growth of SnO2 shells. The thickness of the shell can be simply tailored by controlling the reaction time. Various techniques, including SEM, XRD, TEM and HRTEM, were employed to investigate the morphology, structure and formation process of the special core-shell hollow structures. The combination of magnetic semiconductor (γ-Fe2O3) and wide band-gap semiconductor (SnO2) endowed them with great potential to be used as recyclable photocatalysts. Experiments on photo-degradation of Rhodamin B (RhB) dye in the presence of the samples showed that the hybrid structures possessed higher photocatalytic activities than the monomer structures of SnO2 and γ-Fe2O3 materials indicating a strong coupling enhancement effect between the wide and narrow band-gap semiconductors. Moreover, the gas sensing tests of the γ-Fe2O3@SnO2 hollow NPs revealed that the samples exhibited fast response and recovery rates, which enable them to be promising materials for gas sensors.
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Overexpression of Six1 Gene Suppresses Proliferation and Enhances Expression of Fast-type Muscle Genes in C2C12 Myoblasts
Molecular and Cellular Biochemistry.
Apr, 2013 |
Pubmed ID: 23613228 Sine oculis homeobox 1 (Six1) homeodomain transcription factor is implicated in the genesis of muscle fiber type diversity, but its regulatory mechanisms on the formation of muscle fiber type are still poorly understood. To elucidate the biological roles of Six1 gene in muscle fiber formation, we established C2C12 cell line overexpressing Six1 and determined the effects of forced Six1 expression on muscle-specific genes expression, cell proliferation, and cell cycles. Our results indicated that Six1 overexpression could significantly promote the expression of fast-type muscle genes Atp2a1, Srl, and Mylpf. Furthermore, Six1 overexpressing C2C12 cells displayed a relative lower proliferative potential, and cell cycle analysis showed that Six1 exerted its role in cell cycle primarily through the regulation of G1/S and G2/M phases. In conclusion, Six1 plays an essential role in modulation of the fast-twitch muscle fiber phenotype through up-regulating fast-type muscle genes expression, and it could suppress the proliferation of muscle cells.
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Analysis of the Secretome and Identification of Novel Constituents from Culture Filtrate of Bacillus Calmette-Guerin Using High-resolution Mass Spectrometry
Molecular & Cellular Proteomics : MCP.
Apr, 2013 |
Pubmed ID: 23616670 Tuberculosis (TB) is an infectious bacterial disease that causes morbidity and mortality, especially in developing countries. Although the efficacy against TB has displayed a high degree of variability (0-80%) in different trials, Mycobacterium bovis bacillus Calmette-Guerin (BCG) has been recognized as an important weapon for preventing TB worldwide for over 80 years. Because secreted proteins often play vital roles in the interaction between bacteria and host cells, the secretome of mycobacteria is considered to be an attractive reservoir of potential candidate antigens for the development of novel vaccines and diagnostic reagents. In this study, we performed a proteomic analysis of BCG culture filtrate proteins using SDS-PAGE and high-resolution Fourier transform mass spectrometry. In total, 239 proteins (1555 unique peptides) were identified, including 185 secreted proteins or lipoproteins. Furthermore, 17 novel protein products not annotated in the BCG database were detected and validated by RT-PCR at the transcriptional level. Additionally, the translational start sites of 52 proteins were confirmed, and 22 proteins were validated by extension of the translational start sites based on N-terminus-derived peptides. Compared with previous studies on the mycobacterial secretome, there are 103 secreted proteins that have not been reported that are unique to our study. The physicochemical characteristics of the secreted proteins were determined. Major components from the culture supernatant, including low-molecular-weight antigens, lipoproteins, PE and PPE family proteins and Mce family proteins, were discussed; some components represent potential predominant antigens in the humoral and cellular immune responses.
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The Complete Mitochondrial Genome of Liriomyza Huidobrensis and Comparison with L. Trifolii and L. Sativae (Diptera: Agromyzidae)
Mitochondrial DNA.
Apr, 2013 |
Pubmed ID: 23617371 Abstract Liriomyza huidobrensis, L. trifolii and L. sativae (Diptera: Agromyzidae) are serious horticultural pests. The three species are very similar and difficult to discriminate from each other. In this study, we report the mitochondrial genome of L. huidobrensis and compare with L. trifolii and L. sativae. The mitochondrial genome of L. huidobrensis is 16,239 bp long, and it contains 37 genes and one A + T-rich region as do the sequenced liriomyza species. The sequence and structure of H51-H100 region in srRNA is significantly different from that of L. trifolii and L. sativae. The greatest sequence differences among the three leafminers are located in the region including ND2, ND3, ND6, ATP6, ATP8, CYTb and A+T region, which can be considered as candidate regions for molecular identification or SNP markers.
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Ultrastructure of Germ Cells, Sertoli Cells and Mitochondria During Spermatogenesis in Mature Testis of the Chinese Taihang Black Goats (Capra Hircus)
Micron (Oxford, England : 1993).
Apr, 2013 |
Pubmed ID: 23618746 The objective of this study was to describe the ultrastructure of germ cells, Sertoli cells and mitochondria in mature testis of the Chinese Taihang black goat. The characteristics of germ cell nucleus and mitochondria changing during spermatogenesis were investigated by transmission electron microscopy (TEM). The results showed that the spermatogonium was elliptical, and its nucleus was about 4-5μm. The round mitochondria can be observed throughout the cytoplasm around the nucleus. Small patches of heterochromatin were distributed throughout the nucleus. Spermatocyte was oval-shaped with a nucleus of about 4-4.5μm in diameter. The heterochromatin began to attach to the inner surface of the nuclear membrane. Spermatid was about 4μm and oval in shape. Its nucleus was oval or round and approximately 2-3μm in diameter. The borderline between nucleus membrane and karyoplasm was distinct. During spermiogenesis, spermatid nucleus was condensed and elongated, and chromatin reached the highest condensation in the mature spermatozoon. The mid-piece was surrounded by mitochondria at the neck region. The sperm tail showed the typical "9+2″ structure, contained axoneme and central singlet microtubules. The nuclei of the Sertoli cells were irregular shaped and showed indentations in the membrane. In the mature testes of goat bucks, abundant mitochondria were around the germ cells and Sertoli cells. The scattered mitochondria were aggregated around the base of the flagellum (axoneme) during the spermatid differentiation stage. In conclusion, the present study showed that the spermatogenic process of Taihang black goat followed the pattern of mammals with some specific.
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A B3LYP and MP2(full) Theoretical Investigation into the Cooperativity Effect Between Dihydrogen-bonding and H-M∙∙∙π (M = Li, Na, K) Interactions Among HF, MH with the π-electron Donor C2H 2, C 2H 4 or C 6H 6
Journal of Molecular Modeling.
Apr, 2013 |
Pubmed ID: 23620226 The DFT-B3LYP/6-311++G(3df,2p) and MP2(full)/6-311++G(3df,2p) calculations were carried out on the binary complex formed by HM (M = Li, Na, K) and HF or the π-electron donor (C2H2, C2H4, C6H6), as well as the ternary system FH∙∙∙HM∙∙∙C2H2/C2H4/C6H6. The cooperativity effect between the dihydrogen-bonding and H-M∙∙∙π interactions was investigated. The result shows that the equilibrium distances R H∙∙∙H and R M∙∙∙π in the ternary complex decrease and both the H∙∙∙H and H-M∙∙∙π interactions are strengthened when compared to the corresponding binary complex. The cooperativity effect of the dihydrogen bond on the H-M∙∙∙π interaction is more pronounced than that of the M∙∙∙π bond on the H∙∙∙H interaction. Furthermore, the values of cooperativity effect follow the order of FH∙∙∙HNa∙∙∙π > FH∙∙∙HLi∙∙∙π > FH∙∙∙HK∙∙∙π and FH∙∙∙HM∙∙∙C6H6 > FH∙∙∙HM∙∙∙C2H4 > FH∙∙∙HM∙∙∙C2H2. The nature of the cooperativity effect was revealed by the analyses of the charge of the hydrogen atoms in H∙∙∙H moiety, atom in molecule (AIM) and electron density shifts methods.
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Characterization and Comparative Profiling of MicroRNAs in a Sexual Dimorphism Insect, Eupolyphaga Sinensis Walker
PloS One.
2013 |
Pubmed ID: 23620723 BACKGROUND: MicroRNAs are now recognized as key post-transcriptional regulators in animal ontogenesis and phenotypic diversity. Eupolyphaga sinensis Walker (Blattaria) is a sexually dimorphic insect, which is also an important source of material used in traditional Chinese medicine. The male E. sinensis have shorter lifecycles and go through fewer instars than the female. Furthermore, the males have forewings, while the females are totally wingless. RESULTS: We used the Illumina/Solexa deep sequencing technology to sequence small RNA libraries prepared from the fourth-instar larvae of male and female E. sinensis. 19,097,799 raw reads were yielded in total: 7,817,445 reads from the female library and 11,280,354 from the male, respectively. As a result, we identified 168 known miRNAs belonging to 55 families as well as 204 novel miRNAs. Moreover, 45 miRNAs showed significantly different expression between the female and the male fourth-instar larvae, and we validated 10 of them by Stem-loop qRT-PCR. Some of these differentially expressed miRNAs are related to metamorphosis, development and phenotypic diversity. CONCLUSIONSSIGNIFICANCE: This is the first comprehensive description of miRNAs in E. sinensis. The results provide a useful resource for further in-depth study on molecular regulation and evolution of miRNAs. These findings not only enrich miRNAs for hemimetabolans but also lay the foundation for the study of post-transcriptional regulation on the phenomena of sexual dimorphism.
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Does Chloroplast Size Influence Photosynthetic Nitrogen Use Efficiency?
PloS One.
2013 |
Pubmed ID: 23620801 High nitrogen (N) supply frequently results in a decreased photosynthetic N-use efficiency (PNUE), which indicates a less efficient use of accumulated Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Chloroplasts are the location of Rubisco and the endpoint of CO2 diffusion, and they play a vital important role in photosynthesis. However, the effects of chloroplast development on photosynthesis are poorly explored. In the present study, rice seedlings (Oryza sativa L., cv. 'Shanyou 63', and 'Yangdao 6') were grown hydroponically with three different N levels, morphological characteristics, photosynthetic variables and chloroplast size were measured. In Shanyou 63, a negative relationship between chloroplast size and PNUE was observed across three different N levels. Here, plants with larger chloroplasts had a decreased ratio of mesophyll conductance (gm) to Rubisco content (gm/Rubisco) and a lower Rubisco specific activity. In Yangdao 6, there was no change in chloroplast size and no decline in PNUE or gm/Rubisco ratio under high N supply. It is suggested that large chloroplasts under high N supply is correlated with the decreased Rubisco specific activity and PNUE.
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