Articles by Rodrigo Lopez Gutierrez in JoVE
Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis Alireza Lorzadeh1, Rodrigo Lopez Gutierrez1, Linda Jackson1, Michelle Moksa1, Martin Hirst1,2 1Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology, University of British Columbia, 2Canada's Michael Smith Genome Science Center, BC Cancer Agency We present a modified native chromatin immunoprecipitation sequencing (ChIP-seq) methodology for the generation of sequence datasets suitable for a nucleosome density ChIP-seq analytical framework integrating micrococcal nuclease (MNase) accessibility with histone modification measurements.
Other articles by Rodrigo Lopez Gutierrez on PubMed
Genome-wide Analysis of Cis-regulatory Element Structure and Discovery of Motif-driven Gene Co-expression Networks in Grapevine DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes. | Pubmed ID: 28119334 Coordinated transcriptional and metabolic reprogramming ensures a plant's continued growth and survival under adverse environmental conditions. Transcription factors (TFs) act to modulate gene expression through complex cis-regulatory element (CRE) interactions. Genome-wide analysis of known plant CREs was performed for all currently predicted protein-coding gene promoters in grapevine (Vitis vinifera L.). Many CREs such as abscisic acid (ABA)-responsive, drought-responsive, auxin-responsive, and evening elements, exhibit bona fide CRE properties such as strong position bias towards the transcription start site (TSS) and over-representation when compared with random promoters. Genes containing these CREs are enriched in a large repertoire of plant biological pathways. Large-scale transcriptome analyses also show that these CREs are highly implicated in grapevine development and stress response. Numerous CRE-driven modules in condition-specific gene co-expression networks (GCNs) were identified and many of these modules were highly enriched for plant biological functions. Several modules corroborate known roles of CREs in drought response, pathogen defense, cell wall metabolism, and fruit ripening, whereas others reveal novel functions in plants. Comparisons with Arabidopsis suggest a general conservation in promoter architecture, gene expression dynamics, and GCN structure across species. Systems analyses of CREs provide insights into the grapevine cis-regulatory code and establish a foundation for future genomic studies in grapevine.