Articles by Shahram Jevin Poureetezadi in JoVE
Production of Haploid Zebrafish Embryos by In Vitro Fertilization Paul T. Kroeger Jr.1, Shahram Jevin Poureetezadi1, Robert McKee1, Jonathan Jou1, Rachel Miceli1, Rebecca A. Wingert1 1Department of Biological Sciences, University of Notre Dame The zebrafish is a powerful model system for developmental biology and human disease research due to their genetic similarity with higher vertebrates. This protocol describes a methodology to create haploid zebrafish embryos that can be utilized for forward screen strategies to identify recessive mutations in genes essential for early embryogenesis.
A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos Shahram Jevin Poureetezadi1, Eric K. Donahue1, Rebecca A. Wingert1 1Department of Biological Sciences, University of Notre Dame The zebrafish is an excellent experimental organism to study vertebrate developmental processes and model human disease. Here, we describe a protocol on how to perform a manual high-throughput chemical screen in zebrafish embryos with a whole-mount in situ hybridization (WISH) read-out.
Other articles by Shahram Jevin Poureetezadi on PubMed
Congenital and Acute Kidney Disease: Translational Research Insights from Zebrafish Chemical Genetics General Medicine (Los Angeles, Calif.). Sep, 2013 | Pubmed ID: 24653992 Today, acute kidney injury (AKI) and congenital anomalies of the kidney and urinary tract (CAKUT) represent major issues in healthcare. Both AKI and CAKUT can lead to end stage renal disease (ESRD) that requires life-long medical care with renal replacement therapy. Renal replacement by dialysis is intensive, and kidney transplantation is restricted by organ availability. These limitations, along with the growing epidemic of patients affected by kidney disease, highlight the significant need to identify alternative ways to treat renal injury and birth defects. Drug discovery is one promising avenue of current research. Here, we discuss zebrafish chemical genetics and its latent potency as a method to rapidly identify small molecule therapeutics to accelerate recovery after AKI. Specifically, we review two groundbreaking studies that have recently provided a template to screen for compounds that expand the renal progenitor field in development that were capable of treating AKI in both the zebrafish and the mouse. These new findings demonstrate that drug discovery using zebrafish can be used for relevant translational research to identify clinical interventions for renal conditions in humans.