In JoVE (1)
Other Publications (1)
Articles by Shane Zozula in JoVE
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts Mitchel C. Schiewe1, Shane Zozula1, Nancy Nugent1, Kelley Waggoner1, Jessica Borba1, Lisa Gamboa1, John B. Whitney1 1Ovation Fertility MicroSecure Vitrification was developed as a non-commercial, aseptic closed vitrification device system that is compliant with FDA good manufacturing and tissue-handling practices. Due to the withdrawal of hydrophobic plugged embryo straws from the industry, the vitrification procedure was modified to include an inner seal before the standard internal cotton plug.
Other articles by Shane Zozula on PubMed
Validation of MicroSecure Vitrification (μS-VTF) for the Effective Cryopreservation of Human Embryos and Oocytes Cryobiology. Oct, 2015 | Pubmed ID: 26210008 A novel, aseptic closed system vitrification (VTF) technique for the cryopreservation of embryos and oocytes has been developed and clinically validated in this study. It combines the practicality of embryo-containing sterile flexipettes stored safely and securely with 0.3 ml CBS™ embryo straws possessing weld seals. The cooling and warming rates of this double container system were determined using a data logger. Upon direct plunging into LN(2), the flexipettes cool at an average rate of 1391°C/min, while warming occurs at an average rate of 6233°C/min in a 37°C 0.5 M sucrose bath. Direct deposition of the flexipette into a warming bath insured a rapid transition between -100 and -60°C to minimize potentially harmful recrystalization associated with devitrification. In conclusion, the μS-VTF system has exhibited higher (p