In JoVE (1)
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Articles by Soumili Chatterjee in JoVE
Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels Sandip Basak1, Soumili Chatterjee1, Sudha Chakrapani1,2 1Department of Physiology and Biophysics, Case Western Reserve University, 2School of Medicine, Case Western Reserve University This article describes methods for site-directed spin labeling and reconstitution of pentameric ligand-gated channels for Electron Paramagnetic Resonance studies. This protocol can be adapted for any membrane protein. The reconstitution method described here can also be used for patch-clamp measurements of macroscopic and single-channel currents in a defined lipid system.