In JoVE (1)
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Articles by Sundipta D. Rao in JoVE
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks Yuan-Jyue Chen1, Sundipta D. Rao1, Georg Seelig1,2 1Department of Electrical Engineering, University of Washington, 2Department of Computer Science & Engineering, University of Washington This protocol describes a method for deriving DNA strand displacement gates from plasmids and testing them using fluorescence kinetics measurements. Gates can be modularly composed into multi-component systems to approximate the behavior of formal chemical reaction networks (CRN), demonstrating a new use for CRNs as a molecular programming language.