Articles by Takuma Iguchi in JoVE
Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR Takuma Iguchi1, Noriyo Niino1, Satoshi Tamai1, Ken Sakurai1, Kazuhiko Mori1 1Medicinal Safety Research Laboratories, Daiichi Sankyo Co., Ltd. This report describes a protocol for measuring the absolute levels of plasma miRNA, using quantitative real-time reverse transcription PCR with or without pre-amplification. This protocol affords better understanding of the quantity of plasma miRNAs and allows qualitative assessment of corresponding data from different studies or laboratories.
Other articles by Takuma Iguchi on PubMed
Gene Expression Analysis of Arc MRNA As a Neuronal Cell Activity Marker in the Hippocampus and Amygdala in Two-way Active Avoidance Test in Rats Journal of Pharmacological and Toxicological Methods. Nov - Dec, 2017 | Pubmed ID: 28962918 Immediate early genes are widely used as neuronal cell activity markers in neuroscience. The present study investigated the relationship between their expression and abnormality in context fear conditioning.