Tom F. A. de Greef Department of Biomedical Engineering, Institute for Complex Molecular Systems Eindhoven University of Technology Biography Publications Institution JoVE Articles Tom F. A. de Greef has not added a biography. If you are Tom F. A. de Greef and would like to personalize this page please email our Author Liaison for assistance. Publications Engineering a Scalable and Orthogonal Platform for Synthetic Communication in Mammalian Cells Nature Communications. Nov, 2023 | Pubmed ID: 37919273 Noise Minimization in Cell-Free Gene Expression ACS Synthetic Biology. Aug, 2023 | Pubmed ID: 37478000 DNA Storage in Thermoresponsive Microcapsules for Repeated Random Multiplexed Data Access Nature Nanotechnology. Aug, 2023 | Pubmed ID: 37142708 A Theoretical Framework for Implementable Nucleic Acids Feedback Systems Bioengineering (Basel, Switzerland). Apr, 2023 | Pubmed ID: 37106653 Engineering Cytokine Therapeutics Nature Reviews Bioengineering. 2023 | Pubmed ID: 37064653 Orthogonal Light-Dependent Membrane Adhesion Induces Social Self-Sorting and Member-Specific DNA Communication in Synthetic Cell Communities Small (Weinheim an Der Bergstrasse, Germany). Mar, 2023 | Pubmed ID: 36599623 A Synthetic Protocell-Based Heparin Scavenger Small (Weinheim an Der Bergstrasse, Germany). Mar, 2023 | Pubmed ID: 35570377 A Microfluidic Optimal Experimental Design Platform for Forward Design of Cell-free Genetic Networks Nature Communications. Jun, 2022 | Pubmed ID: 35750678 A Universal Microfluidic Approach for Integrated Analysis of Temporal Homocellular and Heterocellular Signaling and Migration Dynamics Biosensors & Bioelectronics. Sep, 2022 | Pubmed ID: 35594624 Protocellular CRISPR/Cas-Based Diffusive Communication Using Transcriptional RNA Signaling Angewandte Chemie (International Ed. in English). Jun, 2022 | Pubmed ID: 35385207 DNA Input Classification by a Riboregulator-Based Cell-Free Perceptron ACS Synthetic Biology. Apr, 2022 | Pubmed ID: 35381174 A Plug-and-play Platform of Ratiometric Bioluminescent Sensors for Homogeneous Immunoassays Nature Communications. Jul, 2021 | Pubmed ID: 34321486 Determinants of Ligand-Functionalized DNA Nanostructure-Cell Interactions Journal of the American Chemical Society. Jul, 2021 | Pubmed ID: 34180666 Cell-Free Characterization of Coherent Feed-Forward Loop-Based Synthetic Genetic Circuits ACS Synthetic Biology. Jun, 2021 | Pubmed ID: 34061505 Dynamic Protease Activation on a Multimeric Synthetic Protein Scaffold Via Adaptable DNA-Based Recruitment Domains Angewandte Chemie (International Ed. in English). May, 2021 | Pubmed ID: 33725379 Assembly of Dynamic Supramolecular Polymers on a DNA Origami Platform Angewandte Chemie (International Ed. in English). Mar, 2021 | Pubmed ID: 33444471 Light-Activated Signaling in DNA-Encoded Sender-Receiver Architectures ACS Nano. Nov, 2020 | Pubmed ID: 33078948 Designed Asymmetric Protein Assembly on a Symmetric Scaffold Angewandte Chemie (International Ed. in English). Jul, 2020 | Pubmed ID: 32333708 Dynamic Modulation of Proximity-induced Enzyme Activity Using Supramolecular Polymers Chemical Communications (Cambridge, England). May, 2020 | Pubmed ID: 32319466 Proximity-induced Caspase-9 Activation on a DNA Origami-based Synthetic Apoptosome Nature Catalysis. Mar, 2020 | Pubmed ID: 32190819 Engineered Living Materials Based on Adhesin-Mediated Trapping of Programmable Cells ACS Synthetic Biology. Mar, 2020 | Pubmed ID: 32105449 Supramolecular Interactions Between Catalytic Species Allow Rational Control over Reaction Kinetics Chemical Science. Oct, 2019 | Pubmed ID: 31827754 Counterion-Dependent Mechanisms of DNA Origami Nanostructure Stabilization Revealed by Atomistic Molecular Simulation ACS Nano. Sep, 2019 | Pubmed ID: 31502824 Efficient Small-Scale Conjugation of DNA to Primary Antibodies for Multiplexed Cellular Targeting Bioconjugate Chemistry. Sep, 2019 | Pubmed ID: 31438665 Pathway Control in Cooperative Vs. Anti-Cooperative Supramolecular Polymers Angewandte Chemie (International Ed. in English). Aug, 2019 | Pubmed ID: 31119831 DNA-based Communication in Populations of Synthetic Protocells Nature Nanotechnology. 04, 2019 | Pubmed ID: 30833694 Cell-free Microcompartmentalised Transcription-translation for the Prototyping of Synthetic Communication Networks Current Opinion in Biotechnology. Aug, 2019 | Pubmed ID: 30594098 Sigma Factor-Mediated Tuning of Bacterial Cell-Free Synthetic Genetic Oscillators ACS Synthetic Biology. 12, 2018 | Pubmed ID: 30408412 DNA-Based Nanodevices Controlled by Purely Entropic Linker Domains Journal of the American Chemical Society. 11, 2018 | Pubmed ID: 30351025 Model-driven Engineering of Supramolecular Buffering by Multivalency Proceedings of the National Academy of Sciences of the United States of America. 12, 2017 | Pubmed ID: 29158398 Hierarchical Control of Enzymatic Actuators Using DNA-based Switchable Memories Nature Communications. 10, 2017 | Pubmed ID: 29061965 Incorporation of Native Antibodies and Fc-fusion Proteins on DNA Nanostructures Via a Modular Conjugation Strategy Chemical Communications (Cambridge, England). Jun, 2017 | Pubmed ID: 28617516 Non-equilibrium Supramolecular Polymerization Chemical Society Reviews. Sep, 2017 | Pubmed ID: 28349143 Antibody-controlled Actuation of DNA-based Molecular Circuits Nature Communications. 02, 2017 | Pubmed ID: 28211541 Affinity Maturation of a Cyclic Peptide Handle for Therapeutic Antibodies Using Deep Mutational Scanning The Journal of Biological Chemistry. 01, 2017 | Pubmed ID: 27974464 Small-Molecule-Induced and Cooperative Enzyme Assembly on a 14-3-3 Scaffold Chembiochem : a European Journal of Chemical Biology. 02, 2017 | Pubmed ID: 27897387 Modulating the Nucleated Self-Assembly of Tri-β(3) -Peptides Using Cucurbit[n]urils Chemistry (Weinheim an Der Bergstrasse, Germany). Aug, 2016 | Pubmed ID: 27434777 Supramolecular Control over Split-Luciferase Complementation Angewandte Chemie (International Ed. in English). 07, 2016 | Pubmed ID: 27356091 Fragmentation and Coagulation in Supramolecular (Co)polymerization Kinetics ACS Central Science. Apr, 2016 | Pubmed ID: 27163054 A Mix-and-read Drop-based in Vitro Two-hybrid Method for Screening High-affinity Peptide Binders Scientific Reports. Mar, 2016 | Pubmed ID: 26940078 How to Make an Oscillator ELife. Dec, 2015 | Pubmed ID: 26651824 Kinetic Analysis As a Tool to Distinguish Pathway Complexity in Molecular Assembly: An Unexpected Outcome of Structures in Competition Journal of the American Chemical Society. Oct, 2015 | Pubmed ID: 26354151 Programmable Chemical Reaction Networks: Emulating Regulatory Functions in Living Cells Using a Bottom-up Approach Chemical Society Reviews. Nov, 2015 | Pubmed ID: 26214155 Rational Design of Functional and Tunable Oscillating Enzymatic Networks Nature Chemistry. Feb, 2015 | Pubmed ID: 25615670 Supramolecular Buffering by Ring-chain Competition Journal of the American Chemical Society. Feb, 2015 | Pubmed ID: 25581227 Alternation and Tunable Composition in Hydrogen Bonded Supramolecular Copolymers Chemical Communications (Cambridge, England). Mar, 2014 | Pubmed ID: 24452073 Bcrp1;Mdr1a/b;Mrp2 Combination Knockout Mice: Altered Disposition of the Dietary Carcinogen PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) and Its Genotoxic Metabolites Molecular Pharmacology. Mar, 2014 | Pubmed ID: 24334255 Model-driven Optimization of Multicomponent Self-assembly Processes Proceedings of the National Academy of Sciences of the United States of America. Oct, 2013 | Pubmed ID: 24101463 Interaction of 14-3-3 Proteins with the Estrogen Receptor Alpha F Domain Provides a Drug Target Interface Proceedings of the National Academy of Sciences of the United States of America. May, 2013 | Pubmed ID: 23676274 Small Sized Perylene-bisimide Assemblies Controlled by Both Cooperative and Anti-cooperative Assembly Processes Chemical Communications (Cambridge, England). Jun, 2013 | Pubmed ID: 23536123 Evaporative Self-assembly of Single-chain, Polymeric Nanoparticles Chemical Communications (Cambridge, England). Apr, 2013 | Pubmed ID: 23471136 The Origin of Isotope-induced Helical-sense Bias in Supramolecular Polymers of Benzene-1,3,5-tricarboxamides Physical Chemistry Chemical Physics : PCCP. Oct, 2012 | Pubmed ID: 22991036 Symmetry Breaking in the Self-assembly of Partially Fluorinated Benzene-1,3,5-tricarboxamides Angewandte Chemie (International Ed. in English). Nov, 2012 | Pubmed ID: 22936630 Controlling Chemical Self-assembly by Solvent-dependent Dynamics Journal of the American Chemical Society. Aug, 2012 | Pubmed ID: 22808949 Benzene-1,3,5-tricarboxamide: a Versatile Ordering Moiety for Supramolecular Chemistry Chemical Society Reviews. Jul, 2012 | Pubmed ID: 22773107 An Equilibrium Model for Chiral Amplification in Supramolecular Polymers The Journal of Physical Chemistry. B. May, 2012 | Pubmed ID: 22443806 Pathway Complexity in Supramolecular Polymerization Nature. Jan, 2012 | Pubmed ID: 22258506 Theoretical Models of Nonlinear Effects in Two-component Cooperative Supramolecular Copolymerizations Nature Communications. Oct, 2011 | Pubmed ID: 22027589 Controlled Supramolecular Oligomerization of C3-symmetrical Molecules in Water: the Impact of Hydrophobic Shielding Chemistry (Weinheim an Der Bergstrasse, Germany). Apr, 2011 | Pubmed ID: 21432920 Single-chain Folding of Polymers for Catalytic Systems in Water Journal of the American Chemical Society. Apr, 2011 | Pubmed ID: 21405022 Understanding Cooperativity in Hydrogen-bond-induced Supramolecular Polymerization: a Density Functional Theory Study The Journal of Physical Chemistry. B. Nov, 2010 | Pubmed ID: 20942466 Competitive Intramolecular Hydrogen Bonding in Oligo(ethylene Oxide) Substituted Quadruple Hydrogen Bonded Systems The Journal of Organic Chemistry. Feb, 2010 | Pubmed ID: 20063855 Self-assembly of Ureido-pyrimidinone Dimers into One-dimensional Stacks by Lateral Hydrogen Bonding Chemistry (Weinheim an Der Bergstrasse, Germany). Feb, 2010 | Pubmed ID: 20039341 Dynamic Supramolecular Polymers Based on Benzene-1,3,5-tricarboxamides: the Influence of Amide Connectivity on Aggregate Stability and Amplification of Chirality Chemistry (Weinheim an Der Bergstrasse, Germany). Jan, 2010 | Pubmed ID: 20025000 How to Distinguish Isodesmic from Cooperative Supramolecular Polymerisation Chemistry (Weinheim an Der Bergstrasse, Germany). Jan, 2010 | Pubmed ID: 19921721 Supramolecular Polymerization Chemical Reviews. Nov, 2009 | Pubmed ID: 19769364 Photodimerization Processes in Self-assembled Chiral Oligo(p-phenylenevinylene) Bolaamphiphiles Chemistry, an Asian Journal. Jun, 2009 | Pubmed ID: 19360769 Insights into Templated Supramolecular Polymerization: Binding of Naphthalene Derivatives to SsDNA Templates of Different Lengths Journal of the American Chemical Society. Jan, 2009 | Pubmed ID: 19113853 The Influence of Ethylene Glycol Chains on the Thermodynamics of Hydrogen-bonded Supramolecular Assemblies in Apolar Solvents Chemical Communications (Cambridge, England). Sep, 2008 | Pubmed ID: 18802552 Influence of Selectivity on the Supramolecular Polymerization of AB-type Polymers Capable of Both A X A and A X B Interactions Journal of the American Chemical Society. Oct, 2008 | Pubmed ID: 18800796 The Mechanism of Ureido-pyrimidinone:2,7-diamido-naphthyridine Complexation and the Presence of Kinetically Controlled Pathways in Multicomponent Hydrogen-bonded Systems Journal of the American Chemical Society. Apr, 2008 | Pubmed ID: 18373340 Helicity Induction and Amplification in an Oligo(p-phenylenevinylene) Assembly Through Hydrogen-bonded Chiral Acids Angewandte Chemie (International Ed. in English). 2007 | Pubmed ID: 17886328 Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology Maaruthy Yelleswarapu1, Sofia Spinthaki1, Tom F. A. de Greef1, Federica Eduati1 1Department of Biomedical Engineering, Institute for Complex Molecular Systems, Eindhoven University of Technology JoVE 66154 Bioengineering A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression Ardjan J. van der Linden1, Maaruthy Yelleswarapu2, Pascal A. Pieters1, Zoe Swank3, Wilhelm T. S. Huck2, Sebastian J. Maerkl3, Tom F. A. de Greef1,2 1Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group, Eindhoven University of Technology, 2Institute for Molecules and Materials, Radboud University, 3Institute of Bioengineering, School of Engineering École Polytechnique Fédérale de Lausanne (EPFL) JoVE 59655 Bioengineering
Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology Maaruthy Yelleswarapu1, Sofia Spinthaki1, Tom F. A. de Greef1, Federica Eduati1 1Department of Biomedical Engineering, Institute for Complex Molecular Systems, Eindhoven University of Technology JoVE 66154 Bioengineering
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression Ardjan J. van der Linden1, Maaruthy Yelleswarapu2, Pascal A. Pieters1, Zoe Swank3, Wilhelm T. S. Huck2, Sebastian J. Maerkl3, Tom F. A. de Greef1,2 1Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group, Eindhoven University of Technology, 2Institute for Molecules and Materials, Radboud University, 3Institute of Bioengineering, School of Engineering École Polytechnique Fédérale de Lausanne (EPFL) JoVE 59655 Bioengineering
