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A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
This content is Free Access.
1Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group,Eindhoven University of Technology, 2Institute for Molecules and Materials,Radboud University, 3Institute of Bioengineering,School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)
Chapters
- 00:04Title
- 01:00Off-Chip Cooling Setup
- 02:35Preparing the Setup for an Experiment
- 04:20Connecting Uncooled Reagents to the Device
- 05:45Connecting Cooled Fluids to the Microfluidic Device
- 06:49Loading the Microfluidic Device
- 08:09Calibrating the Microfluidic Device
- 08:56Results: IVTT Experiment Expressing the deGFP Protein
- 10:40Conclusion
The fabrication process of a PDMS-based, multilayer, microfluidic device that allows in vitro transcription and translation (IVTT) reactions to be performed over prolonged periods is described. Furthermore, a comprehensive overview of the hardware and software required to automate and maintain these reactions for prolonged durations is provided.
