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JoVE Journal
Bioengineering

This content is Open Access.

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
 

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Article doi: 10.3791/59655
October 6th, 2019

Summary October 6th, 2019

The fabrication process of a PDMS-based, multilayer, microfluidic device that allows in vitro transcription and translation (IVTT) reactions to be performed over prolonged periods is described. Furthermore, a comprehensive overview of the hardware and software required to automate and maintain these reactions for prolonged durations is provided.

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