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Articles by Vikki Marie Weake in JoVE
Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis Jingqun Ma1, Vikki Marie Weake1 1Department of Biochemistry, Purdue University Drosophila tissues often contain a heterogeneous mixture of cell types. To examine gene expression in specific cell types from a particular tissue, nuclei can be genetically tagged and subsequently isolated using an affinity-based approach. Isolated nuclei can be used for downstream applications such as gene expression analysis and chromatin immunoprecipitation.